Usb1 controls U6 snRNP assembly through evolutionarily divergent cyclic phosphodiesterase activities

Allison L. Didychuk; Eric J. Montemayor; Tucker J. Carrocci; Andrew T. DeLaitsch; Stefani E. Lucarelli; William M. Westler; David A. Brow; Aaron A. Hoskins; Samuel E. Butcher

doi:10.1038/s41467-017-00484-w

Nature Communications (Sep 2017)

Usb1 controls U6 snRNP assembly through evolutionarily divergent cyclic phosphodiesterase activities

Allison L. Didychuk,
Eric J. Montemayor,
Tucker J. Carrocci,
Andrew T. DeLaitsch,
Stefani E. Lucarelli,
William M. Westler,
David A. Brow,
Aaron A. Hoskins,
Samuel E. Butcher

Affiliations

Allison L. Didychuk: Department of Biochemistry, University of Wisconsin
Eric J. Montemayor: Department of Biochemistry, University of Wisconsin
Tucker J. Carrocci: Department of Biochemistry, University of Wisconsin
Andrew T. DeLaitsch: Department of Biochemistry, University of Wisconsin
Stefani E. Lucarelli: Department of Biochemistry, University of Wisconsin
William M. Westler: National Magnetic Resonance Facility at Madison, Biochemistry Department, University of Wisconsin-Madison
David A. Brow: Department of Biomolecular Chemistry, University of Wisconsin
Aaron A. Hoskins: Department of Biochemistry, University of Wisconsin
Samuel E. Butcher: Department of Biochemistry, University of Wisconsin

DOI: https://doi.org/10.1038/s41467-017-00484-w
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 13

Abstract

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The mechanism of U6 small nuclear ribonucleoprotein (snRNP) biogenesis is not well understood. Here the authors characterize the enzymatic activities and structures of yeast and human U6 RNA processing enzyme Usb1, reconstitute post-transcriptional assembly of yeast U6 snRNP in vitro, and propose a model for U6 snRNP assembly.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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