PCR-ANALYSIS OF GENOME CHANGEABILITY AND FUSARIUM FUNGI IDENTIFICATION TECHNOLOGY DEVELOPMENT

Abstract

Fusarium moniliforme Sheldon var. lactis intrastrains variability research was carried out. We observed the changes in electrophoretic spectrums of the strains cultures that obtained in result of contact with fusarious resistant and sensible maize genotypes, by different cultivation terms and series passages. At the study of collection and recently isolated F. moniliforme strains intraspecific changeability it was found out that the polymorphism level was 49,4 % and 97,9 %, accordingly. The recently isolated strains clusterization corresponds to plant-owner specialization. It has been developed the PCR-detection system of fusaria with the help of genus-, species- and strain-sequences of DNA nucleotides.

Keywords

• пoлiмepазна ланцюгова реакщя
• fusarium
• ваpiабeльнiсmь геному
• плр-детекція фузарій