Development of Target Quantitative Methods of Flavan-3-ols and their Metabolites
LIU Ruo-nan1,2,
CHEN Wan-bing1,2,
YANG Hong1,
HU Yi-qing3,
LU Qun1,2,
DONG Jun3,
LIU Rui1,2,4
Affiliations
LIU Ruo-nan1,2
1. Key Laboratory of Environment Correlative Dietology, College of Food Science and Technology, Huazhong Agricultural University; 2. Wuhan Engineering Research Center of Bee Products on Quality and Safety Control
CHEN Wan-bing1,2
1. Key Laboratory of Environment Correlative Dietology, College of Food Science and Technology, Huazhong Agricultural University; 2. Wuhan Engineering Research Center of Bee Products on Quality and Safety Control
YANG Hong1
1. Key Laboratory of Environment Correlative Dietology, College of Food Science and Technology, Huazhong Agricultural University
HU Yi-qing3
3. Wuhan Academy of Agricultural Sciences Institute of Environment and Safety
LU Qun1,2
1. Key Laboratory of Environment Correlative Dietology, College of Food Science and Technology, Huazhong Agricultural University; 2. Wuhan Engineering Research Center of Bee Products on Quality and Safety Control
DONG Jun3
3. Wuhan Academy of Agricultural Sciences Institute of Environment and Safety
LIU Rui1,2,4
1. Key Laboratory of Environment Correlative Dietology, College of Food Science and Technology, Huazhong Agricultural University; 2. Wuhan Engineering Research Center of Bee Products on Quality and Safety Control; 4. Key Laboratory of Urban Agriculture in Central China Ministry of Agriculture and Rural Affairs, P. R. China
Flavan-3-ols are important dietary polyphenol, their bioavailability are not only limited to the parent compound, but also the metabolites after the intestinal microbial metabolism in vivo. Aimed to identify the vivo metabolism and the contribution of metabolites to bioavailability, the quantitative analysis methods of dietary flavan-3-ols and their metabolites were researched. According to the difference response value of the product ions of each compound, the appropriate concentration in the mixed standard was determined, and the quantitative analysis method of ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry (UHPLC-QQQ-MS) was established. All compounds had good resolution and appropriate response values within analysis time of 15 min. The method validation results showed that most of the quantitative compounds had good linearity. Urine, feces and plasma were used as samples to investigate the intra-day and the inter-day precision of the method. The RSD value of the method was below 15%, the matrix effect was within ±20%, and the recovery was mainly between 70% and 120%. The method is fast, sensitive, accurate and stable, whicih can be used as a method for targeted quantitative research of flavan-3-ol compounds in vivo.
