Protection effect of Curcumin in ovary culture in NMRI mice

Abstract

Using antioxidants is a known method to increase the number of follicles and ovulation in animals and humans. Curcumin inhibits lipoxygenase, cyclooxygenase. Hence in this study, we examine the effects of different concentrations of curcumin on ovarian tissue culture in NMRI species mice. In this study, 40 varies of female mouse with five weeks ages are transferred to organ culture containers. Studied groups treated by different concentrations of curcumin (10, 20, 40, 80) ng/ml. The ovaries were cultured for 6 days and studied in the case of number of follicles and single layers size and granulosa and analyzed by SPSS software version 20 and ANOVA test. The number of single layer primary follicles in control group (2/3 ± 7/15) and treat groups with 10, 20, 40, 80 ng/ml concentrations of curcumin were (9/5 ± 3/33, 0/5 ± 0/31, 2/4 ± 5/31, 2/3 ± 7/15) respectively which showed a significant increase in all of the treat groups compared with control group (P <0.05). There is also a significant increase in the number of mature follicles in the number of mature follicles cultured in control group (0/4±40) compared with treat group with 10 ng/ml (2/3 ± 5/48). According to the obtained results on the number and quality of follicles, it seem that this method is effective in production, maintain and development of ovarian follicles in culture medium.

Keywords

• tissue culture
• ovarian tissue
• curcumin
• mice nmri