A paired-end whole-genome sequencing approach enables comprehensive characterization of transgene integration in rice

Wenting Xu; Hanwen Zhang; Yuchen Zhang; Ping Shen; Xiang Li; Rong Li; Litao Yang

doi:10.1038/s42003-022-03608-1

Communications Biology (Jul 2022)

A paired-end whole-genome sequencing approach enables comprehensive characterization of transgene integration in rice

Wenting Xu,
Hanwen Zhang,
Yuchen Zhang,
Ping Shen,
Xiang Li,
Rong Li,
Litao Yang

Affiliations

Wenting Xu: National Center for the Molecular Characterization of Genetically Modified Organisms, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University
Hanwen Zhang: National Center for the Molecular Characterization of Genetically Modified Organisms, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University
Yuchen Zhang: National Center for the Molecular Characterization of Genetically Modified Organisms, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University
Ping Shen: Development Center of Science and Technology, Ministry of Agriculture of People’s Republic of China
Xiang Li: Technical Center for Animal, Plant and Food Inspection and Quarantine of Shanghai Customs
Rong Li: National Center for the Molecular Characterization of Genetically Modified Organisms, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University
Litao Yang: National Center for the Molecular Characterization of Genetically Modified Organisms, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University

DOI: https://doi.org/10.1038/s42003-022-03608-1
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 10

Abstract

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Coupling paired-end whole-genome sequencing with droplet digital PCR enabled precise identification of a transgene insertion in the genetically modified rice event G281 on chromosome 3 and the potential for exploring the native gene integration.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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