Ribosome-mediated biosynthesis of pyridazinone oligomers in vitro

Joongoo Lee; Jaime N. Coronado; Namjin Cho; Jongdoo Lim; Brandon M. Hosford; Sangwon Seo; Do Soon Kim; Camila Kofman; Jeffrey S. Moore; Andrew D. Ellington; Eric V. Anslyn; Michael C. Jewett

doi:10.1038/s41467-022-33701-2

Nature Communications (Oct 2022)

Ribosome-mediated biosynthesis of pyridazinone oligomers in vitro

Joongoo Lee,
Jaime N. Coronado,
Namjin Cho,
Jongdoo Lim,
Brandon M. Hosford,
Sangwon Seo,
Do Soon Kim,
Camila Kofman,
Jeffrey S. Moore,
Andrew D. Ellington,
Eric V. Anslyn,
Michael C. Jewett

Affiliations

Joongoo Lee: Department of Chemical and Biological Engineering, Northwestern University
Jaime N. Coronado: Department of Chemistry, University of Texas at Austin
Namjin Cho: Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH)
Jongdoo Lim: Department of Chemistry, University of Texas at Austin
Brandon M. Hosford: Department of Chemistry, University of Texas at Austin
Sangwon Seo: Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST)
Do Soon Kim: Department of Chemical and Biological Engineering, Northwestern University
Camila Kofman: Department of Chemical and Biological Engineering, Northwestern University
Jeffrey S. Moore: Department of Chemistry, University of Illinois at Urbana-Champaign
Andrew D. Ellington: Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas at Austin
Eric V. Anslyn: Department of Chemistry, University of Texas at Austin
Michael C. Jewett: Department of Chemical and Biological Engineering, Northwestern University

DOI: https://doi.org/10.1038/s41467-022-33701-2
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 9

Abstract

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Ribosomes have evolved to polymerize L-α-amino acids into proteins comprising a peptide backbone. Here, a pyridazinone backbone is formed using ribosomes in vitro, producing a variety of sequence-defined alternating block-copolymers.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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