Protocol for ex vivo time lapse imaging of Drosophila melanogaster cytonemes

Guilherme O. Barbosa; Thomas B. Kornberg

STAR Protocols (Mar 2022)

Protocol for ex vivo time lapse imaging of Drosophila melanogaster cytonemes

Guilherme O. Barbosa,
Thomas B. Kornberg

Affiliations

Guilherme O. Barbosa: Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA, USA; Corresponding author
Thomas B. Kornberg: Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA, USA; Corresponding author

Journal volume & issue: Vol. 3, no. 1
p. 101138

Abstract

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Summary: This protocol describes how to image time and spatially resolved time lapses of Drosophila air sac primordium (ASP) cytonemes in ex vivo cultures of wing imaginal discs. It describes how to manually measure the length of cytonemes using custom-made FIJI/ImageJ tools, and to analyze data using R/R-Studios pipeline. It can also be used for studies of cell division, organelle localization, and protein trafficking as well as other cellular materials that can be fluorescently tagged and imaged with minimal phototoxicity.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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