Nature Communications (Jul 2019)

Standard screening methods underreport AAV-mediated transduction and gene editing

  • Jonathan F. Lang,
  • Sushila A. Toulmin,
  • Kasey L. Brida,
  • Laurence C. Eisenlohr,
  • Beverly L. Davidson

DOI
https://doi.org/10.1038/s41467-019-11321-7
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 10

Abstract

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Conventional methods to detect AAV vector transduction can miss transient or low levels of reporter expression. Here the authors use editing-reporter mice and discover numerous sites of AAV targeting along with better prediction of the gene editing footprint.