Targeting specificity of APOBEC-based cytosine base editor in human iPSCs determined by whole genome sequencing

Erica McGrath; Hyunsu Shin; Linyi Zhang; Je-Nie Phue; Wells W. Wu; Rong-Fong Shen; Yoon-Young Jang; Javier Revollo; Zhaohui Ye

doi:10.1038/s41467-019-13342-8

Nature Communications (Nov 2019)

Targeting specificity of APOBEC-based cytosine base editor in human iPSCs determined by whole genome sequencing

Erica McGrath,
Hyunsu Shin,
Linyi Zhang,
Je-Nie Phue,
Wells W. Wu,
Rong-Fong Shen,
Yoon-Young Jang,
Javier Revollo,
Zhaohui Ye

Affiliations

Erica McGrath: Division of Cellular and Gene Therapies, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration
Hyunsu Shin: Division of Cellular and Gene Therapies, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration
Linyi Zhang: Division of Cellular and Gene Therapies, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration
Je-Nie Phue: Facility for Biotechnology Resources, Center for Biologics Evaluation and Research, Food and Drug Administration
Wells W. Wu: Facility for Biotechnology Resources, Center for Biologics Evaluation and Research, Food and Drug Administration
Rong-Fong Shen: Facility for Biotechnology Resources, Center for Biologics Evaluation and Research, Food and Drug Administration
Yoon-Young Jang: Department of Oncology, Johns Hopkins School of Medicine
Javier Revollo: Division of Genetic and Molecular Toxicology, National Center for Toxicology Research, Food and Drug Administration
Zhaohui Ye: Division of Cellular and Gene Therapies, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration

DOI: https://doi.org/10.1038/s41467-019-13342-8
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 9

Abstract

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Cytidine base editors are powerful tools for making subtle genome alterations. Here the authors analyse edited human iPSCs with whole genome sequencing and reveal the spectrum of off-target effects.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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