BMC Genomics (Sep 2009)

Complete genome sequence of the sugarcane nitrogen-fixing endophyte <it>Gluconacetobacter diazotrophicus </it>Pal5

  • Neves Anna,
  • Amaral Gilda,
  • Santos Eidy,
  • Coelho Ana,
  • Sachetto-Martins Gilberto,
  • Alves-Ferreira Marcio,
  • Macedo José,
  • Seibel Luiz,
  • Lemos Melissa,
  • Guillobel Heloisa,
  • Gonzalez Paula,
  • Branco Alan,
  • Ferreira Beatriz,
  • Martin Quintana Flores Victor,
  • de Souza Filho Gonçalo,
  • Junior Jadier,
  • Montano Helena,
  • Figueiredo Daniel,
  • Pozzer Luciana,
  • Meneses Carlos,
  • Brioso Paulo,
  • Rodrigues Elisete,
  • Vidal Marcia,
  • Guedes Helma,
  • Dreschsel Marcela,
  • Freitas Flávia,
  • Valadão Ana,
  • Macedo Jacyara,
  • Simão Tatiana,
  • Oliveira Denise,
  • Cidade Daniela,
  • Nogueira Eduardo,
  • Loureiro Marcio,
  • Almeida Wellington,
  • Cardoso Alexander,
  • Alquéres Sylvia,
  • Magalhães Viviane,
  • França Leonardo,
  • Oliveira André,
  • Araujo Jean,
  • Schwab Stefan,
  • Teixeira Kátia,
  • Hemerly Adriana,
  • Rouws Luc,
  • Rojas Cristian,
  • de Pádua Vânia,
  • Bertalan Marcelo,
  • Albano Rodolpho,
  • Pacheco Ana,
  • Carvalho Daniela,
  • Lery Letícia,
  • Bisch Paulo,
  • Rössle Shaila C,
  • Ürményi Turán,
  • Rael Pereira Alessandra,
  • Silva Rosane,
  • Rondinelli Edson,
  • von Krüger Wanda,
  • Martins Orlando,
  • Baldani José,
  • Ferreira Paulo CG

DOI
https://doi.org/10.1186/1471-2164-10-450
Journal volume & issue
Vol. 10, no. 1
p. 450

Abstract

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Abstract Background Gluconacetobacter diazotrophicus Pal5 is an endophytic diazotrophic bacterium that lives in association with sugarcane plants. It has important biotechnological features such as nitrogen fixation, plant growth promotion, sugar metabolism pathways, secretion of organic acids, synthesis of auxin and the occurrence of bacteriocins. Results Gluconacetobacter diazotrophicus Pal5 is the third diazotrophic endophytic bacterium to be completely sequenced. Its genome is composed of a 3.9 Mb chromosome and 2 plasmids of 16.6 and 38.8 kb, respectively. We annotated 3,938 coding sequences which reveal several characteristics related to the endophytic lifestyle such as nitrogen fixation, plant growth promotion, sugar metabolism, transport systems, synthesis of auxin and the occurrence of bacteriocins. Genomic analysis identified a core component of 894 genes shared with phylogenetically related bacteria. Gene clusters for gum-like polysaccharide biosynthesis, tad pilus, quorum sensing, for modulation of plant growth by indole acetic acid and mechanisms involved in tolerance to acidic conditions were identified and may be related to the sugarcane endophytic and plant-growth promoting traits of G. diazotrophicus. An accessory component of at least 851 genes distributed in genome islands was identified, and was most likely acquired by horizontal gene transfer. This portion of the genome has likely contributed to adaptation to the plant habitat. Conclusion The genome data offer an important resource of information that can be used to manipulate plant/bacterium interactions with the aim of improving sugarcane crop production and other biotechnological applications.