Acinetobacter baumannii biofilm was inhibited by tryptanthrin through disrupting its different stages and genes expression
Tingting Guo,
Na Zhou,
Liying Yang,
Zichen Wang,
Changchao Huan,
Tao Lin,
Guangyu Bao,
Jian Hu,
Guocai Li
Affiliations
Tingting Guo
Department of Microbiology, Medical College, Yangzhou University, Yangzhou 225001, China; Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225001, China; Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou 225001, China
Na Zhou
Department of Microbiology, Medical College, Yangzhou University, Yangzhou 225001, China
Liying Yang
Department of Microbiology, Medical College, Yangzhou University, Yangzhou 225001, China
Zichen Wang
Department of Microbiology, Medical College, Yangzhou University, Yangzhou 225001, China
Changchao Huan
Institute of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou 225001, China
Tao Lin
Department of Laboratory Medicine, Affiliated Hospital, Yangzhou University, Yangzhou 225009, China
Guangyu Bao
Department of Laboratory Medicine, Affiliated Hospital, Yangzhou University, Yangzhou 225009, China
Jian Hu
Department of Laboratory Medicine, Yixing Hospital of Traditional Chinese Medicine/Clinical Medical College, Guangling College, Yangzhou University, Yangzhou 214200, China
Guocai Li
Department of Microbiology, Medical College, Yangzhou University, Yangzhou 225001, China; Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225001, China; Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou 225001, China; Corresponding author
Summary: Biofilm formation plays a significant role in antibiotic resistance, necessitating the search for alternative therapies against biofilm-associated infections. This study demonstrates that 20 μg/mL tryptanthrin can hinder biofilm formation above 50% in various A. baumannii strains. Tryptanthrin impacts various stages of biofilm formation, including the inhibition of surface motility and eDNA release in A. baumannii, as well as an increase in its sensitivity to H202. RT-qPCR analysis reveals that tryptanthrin significantly decreases the expression of the following genes: abaI (19.07%), abaR (33.47%), bfmR (43.41%), csuA/B (64.16%), csuE (50.20%), ompA (67.93%), and katE (72.53%), which are related to biofilm formation and quorum sensing. Furthermore, tryptanthrin is relatively safe and can reduce the virulence of A. baumannii in a Galleria mellonella infection model. Overall, our study demonstrates the potential of tryptanthrin in controlling biofilm formation and virulence of A. baumannii by disrupting different stages of biofilm formation and intercellular signaling communication.
