Development of highly glyphosate-tolerant tobacco by coexpression of glyphosate acetyltransferase gat and EPSPS G2-aroA genes
Baoqing Dun,
Xujing Wang,
Wei Lu,
Ming Chen,
Wei Zhang,
Shuzhen Ping,
Zhixing Wang,
Baoming Zhang,
Min Lin
Affiliations
Baoqing Dun
Institute of Crop Sciences, The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China;Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Xujing Wang
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Wei Lu
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Ming Chen
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Wei Zhang
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Shuzhen Ping
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Zhixing Wang
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Baoming Zhang
Institute of Crop Sciences, The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Min Lin
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
The widely used herbicide glyphosate targets 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). Glyphosate acetyltransferase (GAT) effectively detoxifies glyphosate by N-acetylation. With the aim of identifying a new strategy for development of glyphosate-tolerant crops, the plant expression vector pG2-GAT harboring gat and G2-aroA (encoding EPSPS) has been transformed into tobacco (Nicotiana tabacum) to develop novel plants with higher tolerance to glyphosate. Results from Southern and Western blotting analyses indicated that the target genes were integrated into tobacco chromosomes and expressed effectively at the protein level. Glyphosate tolerance was compared among transgenic tobacco plants containing gat, G2-aroA, or both genes. Plants containing both gat and G2-aroA genes were the most glyphosate-tolerant. This study has shown that a combination of different strategies may result in higher tolerance in transgenic crops, providing a new approach for development of glyphosate-tolerant crops.
