INFLUENCE OF CCC AND TRINEXAPAC-ETHYL ON THE EXPRESSION OF GENES INVOLVED IN GIBBERELLIC BIOSYNTHESIS AND METABOLISM PATHWAY IN ISOGENIC LINE WITH Rht12 DWARFING GENE

Abstract

Occurrence of lodging is a result of environmental stress factors and causes significant losses in crop yields. One of the major factors determining plant resistance to lodging is stem length. Thought, the introduction of the dwarfing genes into genome or application of growth regulators, that inhibit gibberellins biosynthesis, are known as the most important approaches in lodging prevention. In this study we analyzed the influence of chlormequat chloride (CCC) and trinexapac-ethyl application on transcriptome of common wheat (Triticum aestivum L.) ‘Bezostaya 1’. For analysis, the tall control line and isogenic line carrying Rht12 dwarfing gene were selected. Subsequently, the real-time PCR technique was used to determine the expression of five genes encoding enzymes involved in gibberellins biosynthesis pathway (CPS, KS, GA20ox, GA3ox and GA2ox).

Keywords

• common wheat
• lodging
• plant growth regulators
• Rht12 dwarfing gene
• gibberellins biosynthesis pathway