Effect of Lactobacillus salivarius SNK-6 on egg quality, intestinal morphology, and cecal microbial community of laying hens

Wei Liu; Jie Liu; Dapeng Li; Haixia Han; Huaxiang Yan; Yan Sun; Qiuxia Lei; Jie Wang; Yan Zhou; Dingguo Cao; Huimin Li; Fuwei Li

Poultry Science (Jan 2024)

Effect of Lactobacillus salivarius SNK-6 on egg quality, intestinal morphology, and cecal microbial community of laying hens

Wei Liu,
Jie Liu,
Dapeng Li,
Haixia Han,
Huaxiang Yan,
Yan Sun,
Qiuxia Lei,
Jie Wang,
Yan Zhou,
Dingguo Cao,
Huimin Li,
Fuwei Li

Affiliations

Wei Liu: Shandong Academy of Agricultural Sciences, Poultry Institute, 250100, Jinan, China; Poultry Breeding Engineering Technology Center of Shandong Province, 250100, Jinan, China; Jinan Key Laboratory of Poultry Germplasm Resources Innovation and Healthy Breeding, 250100, Jinan, China
Jie Liu: Shandong Academy of Agricultural Sciences, Poultry Institute, 250100, Jinan, China; Poultry Breeding Engineering Technology Center of Shandong Province, 250100, Jinan, China; Jinan Key Laboratory of Poultry Germplasm Resources Innovation and Healthy Breeding, 250100, Jinan, China
Dapeng Li: Shandong Academy of Agricultural Sciences, Poultry Institute, 250100, Jinan, China; Poultry Breeding Engineering Technology Center of Shandong Province, 250100, Jinan, China; Jinan Key Laboratory of Poultry Germplasm Resources Innovation and Healthy Breeding, 250100, Jinan, China
Haixia Han: Shandong Academy of Agricultural Sciences, Poultry Institute, 250100, Jinan, China; Poultry Breeding Engineering Technology Center of Shandong Province, 250100, Jinan, China; Jinan Key Laboratory of Poultry Germplasm Resources Innovation and Healthy Breeding, 250100, Jinan, China
Huaxiang Yan: Shanghai Academy of Agricultural Sciences, Animal Husbandry and Veterinary Research Institute, 201106, Shanghai, China
Yan Sun: Shandong Academy of Agricultural Sciences, Poultry Institute, 250100, Jinan, China; Poultry Breeding Engineering Technology Center of Shandong Province, 250100, Jinan, China; Jinan Key Laboratory of Poultry Germplasm Resources Innovation and Healthy Breeding, 250100, Jinan, China
Qiuxia Lei: Shandong Academy of Agricultural Sciences, Poultry Institute, 250100, Jinan, China; Poultry Breeding Engineering Technology Center of Shandong Province, 250100, Jinan, China; Jinan Key Laboratory of Poultry Germplasm Resources Innovation and Healthy Breeding, 250100, Jinan, China
Jie Wang: Shandong Academy of Agricultural Sciences, Poultry Institute, 250100, Jinan, China; Poultry Breeding Engineering Technology Center of Shandong Province, 250100, Jinan, China; Jinan Key Laboratory of Poultry Germplasm Resources Innovation and Healthy Breeding, 250100, Jinan, China
Yan Zhou: Shandong Academy of Agricultural Sciences, Poultry Institute, 250100, Jinan, China; Poultry Breeding Engineering Technology Center of Shandong Province, 250100, Jinan, China; Jinan Key Laboratory of Poultry Germplasm Resources Innovation and Healthy Breeding, 250100, Jinan, China
Dingguo Cao: Shandong Academy of Agricultural Sciences, Poultry Institute, 250100, Jinan, China; Poultry Breeding Engineering Technology Center of Shandong Province, 250100, Jinan, China; Jinan Key Laboratory of Poultry Germplasm Resources Innovation and Healthy Breeding, 250100, Jinan, China
Huimin Li: Shandong Academy of Agricultural Sciences, Poultry Institute, 250100, Jinan, China; Poultry Breeding Engineering Technology Center of Shandong Province, 250100, Jinan, China; Jinan Key Laboratory of Poultry Germplasm Resources Innovation and Healthy Breeding, 250100, Jinan, China
Fuwei Li: Shandong Academy of Agricultural Sciences, Poultry Institute, 250100, Jinan, China; Poultry Breeding Engineering Technology Center of Shandong Province, 250100, Jinan, China; Jinan Key Laboratory of Poultry Germplasm Resources Innovation and Healthy Breeding, 250100, Jinan, China; Corresponding author:

Journal volume & issue: Vol. 103, no. 1
p. 103224

Abstract

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ABSTRACT: The objective of this study was to investigate the effect of Lactobacillus salivarius (L. salivarius) SNK-6 supple-mentation on the laying performance, egg quality, blood parameters, intestinal morphology, and cecal microbial community of laying hens. A total of 432 healthy 30-wk-age laying hens were randomly divided into 3 groups with 6 replicates under the same husbandry and dietary regimes: control (CON); 2.0 × 108 CFU/kg L. salivarius supplementation (T1); 2.0 × 109 CFU/kg L. salivarius supplementation (T2). The experiment lasted for 10 wk. The results indicated that the supplementation resulted in a significant reduction in the broken egg and unqualified egg ratios, and a significant increase in the eggshell strength, eggshell relative weight, albumen height, and Haugh units (P < 0.05). The L. salivarius-treated hens exhibited significantly reduced serum malondialdehyde levels (P < 0.05); significantly increased total protein, phosphorus, calcitonin, and immunoglobulin M (P < 0.05); significantly increased cecal secretory immunoglobulin A concentration (P < 0.05); significantly improved villus height (VH) in the duodenum and VH to crypt depth ratio in the jejunum (P < 0.05). The serum globulin and interleukin-1β, immunoglobulin G concentrations, and catalase activity significantly increased in T2 (P < 0.05). Furthermore, the serum interferon-α level in T1 was significantly higher than that of the CON (P < 0.05). The intestinal barrier-related mRNA gene ZO-1, CLDN1, and MUC2 expression in the jejunum was significantly upregulated in the T1 and T2 groups (P < 0.05). The Firmicutes/Bacteroidetes ratio was higher and the relative abundances of Flavonifractor and Clostridiales_noname were significantly higher in the T1 group (P < 0.05). In conclusion, dietary supplementation with L. salivarius SNK-6 may improve hen egg quality, serum antioxidant capacity, immune function, and intestinal health.

Published in Poultry Science

ISSN: 0032-5791 (Print); 1525-3171 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Agriculture: Animal culture
Website: https://www.journals.elsevier.com/poultry-science

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