Detecting the DNA of dengue serotype 2 using aluminium nanoparticle doped zinc oxide nanostructure: Synthesis, analysis and characterization

Abstract

Biosensor devices hold great potential for various applications in detecting disease and monitoring disease progression. Electrochemical biosensors represent an important subclass of chemical sensors, they are extremely inexpensive, rapid and sensitive. Aluminium nanoparticle doped zinc oxide nanostructure based integrated electrochemical biosensor has been designed and developed for the dengue virus’s diagnosis. By utilizing a hard mask and thermal evaporator systems (PVD), interdigitated electrode (IDE) is designed with Ag (silver) and integrated on Si-AZO. The platform of fabricated (IDE(Ag)/AZO/Si) is functionalized involving DNA immobilization, hybridization and surface modification to set up electrical detection for DNA probe specific to dengue in concentrations, 100 fM, 100 pM, 10 nM and DNA probe. The sensitivity of wide ranged detected achieved is 55.54 μA nM−1 cm−2, as well as for limit detection, 15.46 μA nM−1 and limit quantification, 51.53 μA nM−1. Also, it is extracted, 0.0604 μA nM−1, the linear regression 0.8563 and the standard deviation of current is 0.34 μA. Our biosensors device is fast and highly effective; thus, it may potentially assist future advances in the diagnosis of dengue viruses.

Keywords

• Interdigitated electrodes
• Immobilization
• DNA probe
• Electrical properties