Frontiers in Microbiology (Dec 2017)
Contribution of RaeB, a Putative RND-Type Transporter to Aminoglycoside and Detergent Resistance in Riemerella anatipestifer
- Xin Zhang,
- Xin Zhang,
- Xin Zhang,
- Ming-Shu Wang,
- Ming-Shu Wang,
- Ming-Shu Wang,
- Ma-Feng Liu,
- Ma-Feng Liu,
- Ma-Feng Liu,
- De-Kang Zhu,
- De-Kang Zhu,
- De-Kang Zhu,
- Francis Biville,
- Ren-Yong Jia,
- Ren-Yong Jia,
- Ren-Yong Jia,
- Shun Chen,
- Shun Chen,
- Shun Chen,
- Kun-Feng Sun,
- Kun-Feng Sun,
- Kun-Feng Sun,
- Qiao Yang,
- Qiao Yang,
- Qiao Yang,
- Ying Wu,
- Ying Wu,
- Ying Wu,
- Xin-Xin Zhao,
- Xin-Xin Zhao,
- Xin-Xin Zhao,
- Xiao-Yue Chen,
- An-Chun Cheng,
- An-Chun Cheng,
- An-Chun Cheng
Affiliations
- Xin Zhang
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Xin Zhang
- Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Xin Zhang
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, China
- Ming-Shu Wang
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Ming-Shu Wang
- Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Ming-Shu Wang
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, China
- Ma-Feng Liu
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Ma-Feng Liu
- Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Ma-Feng Liu
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, China
- De-Kang Zhu
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- De-Kang Zhu
- Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- De-Kang Zhu
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, China
- Francis Biville
- Unité des Infections Bactériennes Invasives, Institut Pasteur, Paris, France
- Ren-Yong Jia
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Ren-Yong Jia
- Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Ren-Yong Jia
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, China
- Shun Chen
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Shun Chen
- Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Shun Chen
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, China
- Kun-Feng Sun
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Kun-Feng Sun
- Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Kun-Feng Sun
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, China
- Qiao Yang
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Qiao Yang
- Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Qiao Yang
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, China
- Ying Wu
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Ying Wu
- Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Ying Wu
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, China
- Xin-Xin Zhao
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Xin-Xin Zhao
- Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- Xin-Xin Zhao
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, China
- Xiao-Yue Chen
- Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- An-Chun Cheng
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- An-Chun Cheng
- Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, China
- An-Chun Cheng
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, China
- DOI
- https://doi.org/10.3389/fmicb.2017.02435
- Journal volume & issue
-
Vol. 8
Abstract
Riemerella anatipestifer is an important pathogenic bacterium that infects ducks. It exhibits resistance to multiple classes of antibiotics. Multidrug efflux pumps play a major role as a mechanism of antimicrobial resistance in Gram-negative pathogens and they are poorly understood in R. anatipestifer. In this study, a gene encoding the B739_0873 protein in R. anatipestifer CH-1, which belongs to the resistance-nodulation-cell division (RND) efflux pump family, was identified. With respect to the substrate specificity of B739_0873, the antibiotic susceptibility testing showed that the B739_0873 knockout strain was more sensitive to aminoglycosides and detergents than the wild-type strain. The transcription of B739_0873 was up-regulated when R. anatipestifer CH-1 was exposed to sub-inhibitory levels of these substrates. From the gentamicin accumulation assay, we concluded that B739_0873 was coupled to the proton motive force to pump out gentamicin. Furthermore, site-directed mutagenesis demonstrated that Asp 400, Asp 401, Lys 929, Arg 959, and Thr 966 were the crucial function sites of B739_0873 in terms of its ability to extrude aminoglycosides and detergents. Finally, we provided evidence that B739_0873 is co-transcribed with B739_0872, and that both B739_0872 and B739_0873 are required for aminoglycoside and detergent resistance. In view of these results, we designate B739_0873 as RaeB (Riemerella anatipestifer efflux).
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