This article introduced the design and application of the ultrasound probe holder for ultrasound-guided venipuncture. The ultrasound probe holder has been designed to hold a variety of probes in different angles, and to provide more comfort with less fatigue for the operator. The components of the probe holder can be dismantled for cleaning or sterilization. (本文介绍一种用于超声引导静脉穿刺的超声探头固定装置的设计与应用。该装置操作简便, 能够灵活调整并固定超声探头, 提高操作者舒适度, 且可拆卸进行清洗或消毒。)