Biocompatible Magnetic Colloidal Suspension Used as a Tool for Localized Hyperthermia in Human Breast Adenocarcinoma Cells: Physicochemical Analysis and Complex In Vitro Biological Profile
Elena-Alina Moacă,
Claudia-Geanina Watz,
Vlad Socoliuc,
Roxana Racoviceanu,
Cornelia Păcurariu,
Robert Ianoş,
Simona Cîntă-Pînzaru,
Lucian Barbu Tudoran,
Fran Nekvapil,
Stela Iurciuc,
Codruța Șoica,
Cristina-Adriana Dehelean
Affiliations
Elena-Alina Moacă
Faculty of Pharmacy, Victor Babeș University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, RO-300041 Timisoara, Romania
Claudia-Geanina Watz
Faculty of Pharmacy, Victor Babeș University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, RO-300041 Timisoara, Romania
Vlad Socoliuc
Romanian Academy—Timisoara Branch, Center for Fundamental and Advanced Technical Research, Laboratory of Magnetic Fluids, 24 M. Viteazu Ave., RO-300223 Timisoara, Romania
Roxana Racoviceanu
Faculty of Pharmacy, Victor Babeș University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, RO-300041 Timisoara, Romania
Cornelia Păcurariu
Faculty of Industrial Chemistry and Environmental Engineering, Politehnica University of Timisoara, 2nd Victoriei Square, RO-300223 Timisoara, Romania
Robert Ianoş
Faculty of Industrial Chemistry and Environmental Engineering, Politehnica University of Timisoara, 2nd Victoriei Square, RO-300223 Timisoara, Romania
Electron Microscopy Laboratory “Prof. C. Craciun”, Faculty of Biology & Geology, “Babes-Bolyai” University, 5-7 Clinicilor Street, RO-400006 Cluj-Napoca, Romania
Department of Cardiology, Faculty of Medicine, Victor Babes University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, RO-300041 Timisoara, Romania
Codruța Șoica
Faculty of Pharmacy, Victor Babeș University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, RO-300041 Timisoara, Romania
Cristina-Adriana Dehelean
Faculty of Pharmacy, Victor Babeș University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, RO-300041 Timisoara, Romania
Magnetic iron oxide nanoparticles are the most desired nanomaterials for biomedical applications due to their unique physiochemical properties. A facile single-step process for the preparation of a highly stable and biocompatible magnetic colloidal suspension based on citric-acid-coated magnetic iron oxide nanoparticles used as an effective heating source for the hyperthermia treatment of cancer cells is presented. The physicochemical analysis revealed that the magnetic colloidal suspension had a z-average diameter of 72.7 nm at 25 °C with a polydispersity index of 0.179 and a zeta potential of −45.0 mV, superparamagnetic features, and a heating capacity that was quantified by an intrinsic loss power analysis. Raman spectroscopy showed the presence of magnetite and confirmed the presence of citric acid on the surfaces of the magnetic iron oxide nanoparticles. The biological results showed that breast adenocarcinoma cells (MDA-MB-231) were significantly affected after exposure to the magnetic colloidal suspension with a concentration of 30 µg/mL 24 h post-treatment under hyperthermic conditions, while the nontumorigenic (MCF-10A) cells exhibited a viability above 90% under the same thermal setup. Thus, the biological data obtained in the present study clearly endorse the need for further investigations to establish the clinical biological potential of synthesized magnetic colloidal suspension for magnetically triggered hyperthermia.