Effect of dietary lycopene supplementation on growth, antioxidant and immunity of juvenile hybrid grouper (♀Epinephelus fuscoguttatus ×♂E. lanceolatus) fed with high lipid diets

Menglong Zhou; Hao Liu; Baiquan Lu; Biao Li; Weibin Huang; Hengyang Song; Wenshan Cai; Beiping Tan; Yuanzhi Yang; Xiaohui Dong

Aquaculture Reports (Dec 2024)

Effect of dietary lycopene supplementation on growth, antioxidant and immunity of juvenile hybrid grouper (♀Epinephelus fuscoguttatus ×♂E. lanceolatus) fed with high lipid diets

Menglong Zhou,
Hao Liu,
Baiquan Lu,
Biao Li,
Weibin Huang,
Hengyang Song,
Wenshan Cai,
Beiping Tan,
Yuanzhi Yang,
Xiaohui Dong

Affiliations

Menglong Zhou: Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Engineering Technology Research Center of Aquatic Animals Precision Nutrition and High Efficiency Feed, Zhanjiang, Guangdong 524088, China
Hao Liu: Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Engineering Technology Research Center of Aquatic Animals Precision Nutrition and High Efficiency Feed, Zhanjiang, Guangdong 524088, China
Baiquan Lu: Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Engineering Technology Research Center of Aquatic Animals Precision Nutrition and High Efficiency Feed, Zhanjiang, Guangdong 524088, China
Biao Li: Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Engineering Technology Research Center of Aquatic Animals Precision Nutrition and High Efficiency Feed, Zhanjiang, Guangdong 524088, China
Weibin Huang: Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Engineering Technology Research Center of Aquatic Animals Precision Nutrition and High Efficiency Feed, Zhanjiang, Guangdong 524088, China
Hengyang Song: Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Engineering Technology Research Center of Aquatic Animals Precision Nutrition and High Efficiency Feed, Zhanjiang, Guangdong 524088, China
Wenshan Cai: Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Engineering Technology Research Center of Aquatic Animals Precision Nutrition and High Efficiency Feed, Zhanjiang, Guangdong 524088, China
Beiping Tan: Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Engineering Technology Research Center of Aquatic Animals Precision Nutrition and High Efficiency Feed, Zhanjiang, Guangdong 524088, China; Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, Guangdong 524000, China; Correspondence to: Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China.
Yuanzhi Yang: Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Engineering Technology Research Center of Aquatic Animals Precision Nutrition and High Efficiency Feed, Zhanjiang, Guangdong 524088, China
Xiaohui Dong: Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Engineering Technology Research Center of Aquatic Animals Precision Nutrition and High Efficiency Feed, Zhanjiang, Guangdong 524088, China; Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, Guangdong 524000, China; Correspondence to: Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China.

Journal volume & issue: Vol. 39
p. 102367

Abstract

Read online

The aim of this experiment was to investigate the effects of dietary lycopene (LCP) on growth performance, antioxidant, and immunity of hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂) fed with high-lipid diets (HLD, CL16 %). Hybrid groupers (20.56±0.17 g) were selected and fed four diets containing 0, 100, 200 and 400 mg/kg of LCP, named L0, L1, L2 and L3. The results showed that: FBW and SGR were significantly higher in all dietary LCP group compared to the L0 group (P< 0.05). In serum, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) content were significantly higher in group L2 than in group L0, and alkaline phosphatase (AKP) content in L3 group was significantly lower (P< 0.05). In the liver, malondialdehyde (MDA) content were significantly lower in all LCP groups than in the L0 group (P< 0.05); catalase (CAT), AKP, and acid phosphatase (ACP) activities were significantly higher in all LCP group than in the L0 group (P< 0.05); SOD, Aspartate aminotransferase (GOT) and glutamate-pyruvate transaminase (GPT) activities in the liver were significantly higher in the L2 group than in the L0 group (P< 0.05). Dietary supplementation of 200–400 mg/kg LCP significantly up-regulated mRNA levels of hepatic sod, gsh-px, cat, nuclear factor erythroid 2-related factor 2 (nrf2), heat stimulating protein 90 (hsp90) and transforming growth factor-β (tgfβ), and significantly down-regulated mRNA levels of hepatic kelch-like ECH-associated protein 1 (keap1) and interleukin-1β (il1β) (P< 0.05). The optimal levels of LCP were 282.22 mg/kg, 246.83 mg/kg and 248.5 mg/kg for FBW, serum GSH-Px activity and hepatic immunoglobulin M (IgM) content, respectively. In conclusion, the addition of 200–300 mg/kg LCP could promote the growth performance of hybrid grouper, improve the antioxidant capacity, and alleviate the oxidative damage of liver caused by HLD.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

About the journal

Abstract

Keywords