Transcriptome analysis of the bipotential gonads of hermaphroditic bivalve species Tridacna crocea unravels the potential genes responsible for spermatogenesis

Zohaib Noor; Zhen Zhao; Shuming Guo; Yanping Qin; Haitao Ma; Ziniu Yu; Jun Li; Yuehuan Zhang

Aquaculture Reports (Jun 2024)

Transcriptome analysis of the bipotential gonads of hermaphroditic bivalve species Tridacna crocea unravels the potential genes responsible for spermatogenesis

Zohaib Noor,
Zhen Zhao,
Shuming Guo,
Yanping Qin,
Haitao Ma,
Ziniu Yu,
Jun Li,
Yuehuan Zhang

Affiliations

Zohaib Noor: Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; University of Chinese Academy of Sciences, Beijing 100049, China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China; Hainan Provincial Key Laboratory of Tropical Marine Biology Technology, Sanya Institute of Oceanology Chinese Academy of Sciences, Sanya 572024, China; Daya Bay Marine Biology Research Station, Chinese Academy of Sciences, Shenzhen 518124, China
Zhen Zhao: Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China; Hainan Provincial Key Laboratory of Tropical Marine Biology Technology, Sanya Institute of Oceanology Chinese Academy of Sciences, Sanya 572024, China; Daya Bay Marine Biology Research Station, Chinese Academy of Sciences, Shenzhen 518124, China
Shuming Guo: Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; University of Chinese Academy of Sciences, Beijing 100049, China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China; Hainan Provincial Key Laboratory of Tropical Marine Biology Technology, Sanya Institute of Oceanology Chinese Academy of Sciences, Sanya 572024, China; Daya Bay Marine Biology Research Station, Chinese Academy of Sciences, Shenzhen 518124, China
Yanping Qin: Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China; Hainan Provincial Key Laboratory of Tropical Marine Biology Technology, Sanya Institute of Oceanology Chinese Academy of Sciences, Sanya 572024, China; Daya Bay Marine Biology Research Station, Chinese Academy of Sciences, Shenzhen 518124, China
Haitao Ma: Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China; Hainan Provincial Key Laboratory of Tropical Marine Biology Technology, Sanya Institute of Oceanology Chinese Academy of Sciences, Sanya 572024, China; Daya Bay Marine Biology Research Station, Chinese Academy of Sciences, Shenzhen 518124, China
Ziniu Yu: Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; University of Chinese Academy of Sciences, Beijing 100049, China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China; Hainan Provincial Key Laboratory of Tropical Marine Biology Technology, Sanya Institute of Oceanology Chinese Academy of Sciences, Sanya 572024, China; Daya Bay Marine Biology Research Station, Chinese Academy of Sciences, Shenzhen 518124, China
Jun Li: Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China; Hainan Provincial Key Laboratory of Tropical Marine Biology Technology, Sanya Institute of Oceanology Chinese Academy of Sciences, Sanya 572024, China; Daya Bay Marine Biology Research Station, Chinese Academy of Sciences, Shenzhen 518124, China; Correspondence to: South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China.
Yuehuan Zhang: Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China; Hainan Provincial Key Laboratory of Tropical Marine Biology Technology, Sanya Institute of Oceanology Chinese Academy of Sciences, Sanya 572024, China; Daya Bay Marine Biology Research Station, Chinese Academy of Sciences, Shenzhen 518124, China; Correspondence to: South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China.

Journal volume & issue: Vol. 36
p. 102039

Abstract

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Sex is an integral part of the evolution of species and often takes the form of intersex and sexual change in mollusks. As a major member of the deep-sea coral reef ecosystem, Tridacna crocea has complex gonadal development mechanisms and reproductive strategies, and the regulation model of spermatogenesis needs to be further explored. In this study, gonadal transcripts of T. crocea in resting, spermatogonia and sperm stage were evaluated. According to the statistics of bioinformatics analysis, a total of 10,260 DEGs were identified, including 3021 genes shared by three developmental stages. Through dynamic expression analysis, we identified the expression patterns and functional classification of genes that regulate spermatogenesis. These candidate genes were involved in regulatory mechanisms such as nucleotide binding, cell cycle, sexual reproduction and DNA replication. Subsequently, based on functional screening, PPI analysis was performed on the target gene cluster to learn the network relationships among candidate genes and other genes closely related to candidate genes. The results showed that in addition to candidate genes (zmcm6, gins2, mcm9, blm, mcm4, psmc3ip, haus, ccnb3, spice, rad1, rad17, ncapg, bub1b, lin9, tipin, and cks), several genes that have been shown to be associated with gonadal development were present in the network (such as fanci, aurka, kif20a and nek2), suggesting the reliability of the results. Finally, the expression levels of candidate genes between individuals at different developmental stages were compared with transcriptional expression profiles, which further proved that these candidate genes were related to T. crocea gonad development traits. These results make up for the lack of research on T. crocea gametogenesis, spermatogenesis and gonad development, and provide a reference for the research on Tridacna reproductive mechanism.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

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