Purification of FLAG-tagged Secreted Proteins from Mammalian Cells

Eisuke Itakura; Changchun Chen; Mario de Bono

doi:10.21769/BioProtoc.2430

Bio-Protocol (Aug 2017)

Purification of FLAG-tagged Secreted Proteins from Mammalian Cells

Eisuke Itakura,
Changchun Chen,
Mario de Bono

Affiliations

Eisuke Itakura: Department of Biology, Graduate School of Science, Chiba University, Chiba, Japan
Changchun Chen: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, UK
Mario de Bono: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, UK

DOI: https://doi.org/10.21769/BioProtoc.2430
Journal volume & issue: Vol. 7, no. 15

Abstract

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This protocol describes a method for purifying glycosylated FLAG-tagged secreted proteins with disulfide bonds from mammalian cells. The purified products can be used for various applications, such as ligand binding assays.

Published in Bio-Protocol

ISSN: 2331-8325 (Online)
Publisher: Bio-protocol LLC
Country of publisher: United States
LCC subjects: Science: Biology (General)
Website: https://bio-protocol.org

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