Physicochemical properties and biocompatibility of the bi-layer polyvinyl alcohol-based hydrogel for osteochondral tissue engineering

Weiwei Lan; Mengjie Xu; Miao Qin; Yizhu Cheng; Ya Zhao; Di Huang; Xiaochun Wei; Yongchang Guo; Weiyi Chen

Materials & Design (Jun 2021)

Physicochemical properties and biocompatibility of the bi-layer polyvinyl alcohol-based hydrogel for osteochondral tissue engineering

Weiwei Lan,
Mengjie Xu,
Miao Qin,
Yizhu Cheng,
Ya Zhao,
Di Huang,
Xiaochun Wei,
Yongchang Guo,
Weiyi Chen

Affiliations

Weiwei Lan: Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China; Shanxi Key Laboratory of Material Strength & Structural Impact, Institute of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China
Mengjie Xu: Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China
Miao Qin: Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China
Yizhu Cheng: Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China
Ya Zhao: Laboratory of Biomaterial Surfaces & Interfaces, Institute of New Carbon Materials, Taiyuan University of Technology, Taiyuan 030024, China
Di Huang: Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China; Shanxi Key Laboratory of Material Strength & Structural Impact, Institute of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China; Corresponding authors at: Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China.
Xiaochun Wei: Department of Orthopaedics, The Second Hospital of Shanxi Medical University, Taiyuan 030001, PR China
Yongchang Guo: Experimental Animal Center of Shanxi Medical University, Taiyuan 030001, PR China
Weiyi Chen: Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China; Shanxi Key Laboratory of Material Strength & Structural Impact, Institute of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China; Corresponding authors at: Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China.

Journal volume & issue: Vol. 204
p. 109652

Abstract

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Osteochondral defect regeneration was a great challenge because of limited self-repaired ability for its sophisticated hierarchical inner structure. Hereon, the bi-layer hydrogel with robust osteochondral interfacial bonding was formulated under fully physical crosslinking, which was consisted of polyvinyl alcohol/collagen II for cartilage layer and polyvinyl alcohol/biphasic calcium phosphate/carbon nanotubes for bone layer. The bi-layer hydrogel with irregular pore distribution showed decreased pore size from top to bottom. The tensile modulus of bi-layer hydrogel reached to 7.14 ± 3 MPa and the stretched fracture surfaces were always appeared in the upper hydrogels, proving bi-layer hydrogels' well interfacial bonding strength. Furthermore, hydrogels' biocompatibility was evaluated using two kinds of cells in vitro, and the repair performance was also assessed in vivo test. The results demonstrated the bi-layer hydrogel owned favorable cytocompatibility and could induce the formation of osteochondral regeneration. Additionally, the max load at defect section reached to 67.24 ± 36.06 N after 12 weeks regeneration, verifying well bonding strength between the neotissue and native adjacent tissues. This work revealed the favorable bi-layer hydrogel owed great potential in repairing osteochondral defects.

Published in Materials & Design

ISSN: 0264-1275 (Print); 1873-4197 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials
Website: https://www.journals.elsevier.com/materials-and-design

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