In vitro cytotoxicity against K562 tumor cell line, antibacterial, antioxidant, antifungal and catalytic activities of biosynthesized silver nanoparticles using Sophora pachycarpa extract

Abstract

In the present study, we demonstrate the green synthesis of silver nanoparticles using Sophora pachycarpa extract (S. pachycarpa; SPE) as capping, reducing, and stabilizing agents. The biosynthesized silver nanoparticles (SPE-AgNPs) were tested for catalytic, antibacterial, antifungal, antioxidant, and anti-cancer activities. The affecting parameters (the concentration of silver nitrate, the temperature of the reaction, and time of reaction) on the synthesis process were optimized. The biosynthesized SPE-AgNPs were studied by X-Ray diffraction (XRD), transmission electron microscopy (TEM), field emission scanning electron microscopy (FESEM), dynamic light scattering (DLS), energy-dispersive X-ray spectroscopy (EDS) and Fourier-transform infrared spectroscopy (FT-IR). The FESEM and TEM results revealed spherical and oval-like morphology with sizes ranging from 30 to 40 nm. Photocatalytic performance experiments of SPE-AgNPs were determined by the rapid degradation of the eriochrome black T (EBT) and methylene blue (MB) under sunlight and UV irradiations. The results showed that SPE-AgNPs degraded more than 90% and 80% of both dyes under UV and sunlight irradiations, respectively. In addition, the SPE-AgNPs exhibited good antibacterial and antifungal properties against S. aureus, S. epidermidis, P. aeruginosa, E. coli, K. pneumoniae, E. faecalis, and C. albicans with MIC values of 6.25, 6.25, 0.78, 0.39, 0.78, 1.56 and 0.78 µg/ml. The green synthesized SPE-AgNPs were found to inhibit the activity of DPPH free radicals efficiently. Eventually, the SPE-AgNPs exhibited significant in vitro cytotoxicity against K562 tumor cell line (IC50 = 19.5 µg/ml). All these studies indicated that AgNPs synthesized using S. pachycarpa extract have applications in the environmental and biomedical fields.

Keywords

• Green synthesis
• Sophora pachycarpa
• Nanoparticle
• Anti-cancer
• Photocatalytic degradation