Diabetes, Metabolic Syndrome and Obesity (Jun 2023)
Combined Exercise Training Promotes More Benefits on Cardiovascular Autonomic Modulation in Ovariectomized Rats Than Isolated Aerobic or Resistance Training
Abstract
Nicolas Da Costa-Santos,1 Gabrielly Minguta Santos Costa,2 Adriano Dos-Santos,1 Bruno Nascimento-Carvalho,3 Thayna Fabiana Ribeiro,1 Sarah Cristina Ferreira Freitas,2 Erico Caperuto,1 Maria-Claudia Irigoyen,3 Katia De Angelis,4 Kátia Bilhar Scapini,1 Iris Callado Sanches1 1Human Movement Laboratory, São Judas University, São Paulo, Brazil; 2Laboratory of Translational Physiology, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil; 3Hypertension Unit, Heart Institute, Medicine School, University of São Paulo, São Paulo, Brazil; 4Physiology Exercise Laboratory, Department of Physiology, Federal University of Sao Paulo (UNIFESP), São Paulo, BrazilCorrespondence: Iris Callado Sanches, Human Movement Laboratory, São Judas University, Rua taquari, 546, São Paulo, SP, 03166-000, Brazil, Email [email protected]: Cardiovascular risk increase after ovarian deprivation has been extensively demonstrated by our research group through cardiovascular autonomic analysis. Interventions involving different types of exercises, such as resistance exercises or combined exercises (aerobic and resistance) have been widely recommended to prevent or minimize neuromuscular decline in postmenopausal women, which is aggravated by a sedentary lifestyle. Experimentally, the cardiovascular effects of resistance or combined training, as well as comparison between aerobic, resistance, and combined training, in ovariectomized animals are scarce.Purpose: In this study, we hypothesized that the combination of aerobic and resistance training may be more effective in preventing muscle mass loss, as well as improving cardiovascular autonomic modulation and baroreflex sensitivity, than aerobic or resistance training individually in ovariectomized rats.Animals and Methods: Female rats were divided into 5 groups: sedentary (C); ovariectomized (Ovx); trained ovariectomized submitted to aerobic training (OvxAT); resistance training (OvxRT); combined training (OvxCT). Exercise training lasted 8 weeks, with the combined group alternating between aerobic training and resistance training every other day. At the end of the study, glycemia and insulin tolerance were evaluated. Arterial pressure (AP) was directly recorded. Baroreflex sensitivity was assessed by heart rate response to changes in arterial pressure. Cardiovascular autonomic modulation was evaluated by spectral analysis.Results: Combined training was the only training regime that increased baroreflex sensitivity for tachycardic response and reduced all systolic blood pressure variability parameters. Furthermore, all animals submitted to exercise training on a treadmill (OvxAT and OvxCT) presented lower systolic, diastolic, and mean pressure, as well as improvements in the autonomic modulation for the heart.Conclusion: Combined training showed to be more effective than isolated aerobic and resistance training, mixing the isolated benefits of each modality. It was the only modality able to increase baroreflex sensitivity to tachycardic responses, reduce arterial pressure and all parameters of vascular sympathetic modulation.Keywords: combined exercise training, resistance exercise training, aerobic exercise training, ovarian hormone deprivation, cardiovascular autonomic modulation, baroreflex sensitivity
