Frontiers in Cell and Developmental Biology (Sep 2021)

Circular RNA CircPPP1CB Suppresses Tumorigenesis by Interacting With the MiR-1307-3p/SMG1 Axis in Human Bladder Cancer

  • Feifan Wang,
  • Yan Zhang,
  • Xuejian Zhou,
  • Xianwu Chen,
  • Jiayong Xiang,
  • Mengjing Fan,
  • Yanlan Yu,
  • Yueshu Cai,
  • Hongshen Wu,
  • Shihan Huang,
  • Ning He,
  • Zhenghui Hu,
  • Guoqing Ding,
  • Xiaodong Jin

DOI
https://doi.org/10.3389/fcell.2021.704683
Journal volume & issue
Vol. 9

Abstract

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Circular RNA (circRNA) is a newly discovered endogenous non-coding RNA (ncRNA), which is characterized with a closed circular structure. A growing body of evidence has verified the vital roles of circRNAs in human cancer. In this research, we selected circPPP1CB as a study object by circRNA sequencing and quantitative real-time PCR (qRT-PCR) validation in human bladder cancer (BC). CircPPP1CB is downregulated in BC and is negatively correlated with clinical stages and histological grades. Functionally, circPPP1CB modulated cell growth, metastasis, and epithelial-to-mesenchymal transition (EMT) process in vitro and in vivo. Mechanically, we performed various experiments to verify the circPPP1CB/miR-1307-3p/SMG1 regulatory axis. Taken together, our results demonstrated that circPPP1CB participates in tumor growth, metastasis, and EMT process by interacting with the miR-1307-3p/SMG1 axis, and that circPPP1CB might be a novel therapeutic target and diagnostic biomarker in human BC.

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