Ultrasensitive immunochromatographic strip for detection of cyproheptadine

Abstract

Using immunological and cell fusion technology, group-specific monoclonal antibodies were produced against cyproheptadine. The 50% inhibitory concentration of the antibody for cyproheptadine was 0.37 ng/mL, the linear range was 0.16–0.85 ng/mL, and recoveries of antibody in spiked negative pig urine were 80–120%. An ultrasensitive immunochromatographic strip, based on the antibody, was developed for the rapid detection of cyproheptadine in pig urine. The test strip had a cut-off value of 5 ng/mL.

Keywords

• cyproheptadine
• indirect competitive enzyme-linked immunosorbent assay (ic-elisa)
• immunochromatographic test strip
• urine