PREPARASI NANOPARTIKEL KITOSAN-TPP/ EKSTRAK ETANOL DAGING BUAH MAHKOTA DEWA (Phaleriamacrocarpa (Scheff) Boerl) DENGAN METODE GELASI IONIK

Abstract

Abstract: Mahkota dewa (Phaleria macrocarpa (Scheff) Boerl) is one of the plants frequently used as anticancer. But mahkota dewa extract have a high toxicity. One of certain effort that could reduces the toxic effect of medicine is nanoparticle decivery. The aims of this study were to make nanoparticle from chitosan-mahkota dewa extract using TPP as a cross linker and to determine it's particle size, zeta potential, loading capacity and loading efficiency. Chitosan nanoparticles extract of Phaleria macrocarpa fruits with TPP as cross linker by ionic gelation method. Chitosan was dissolved in acetic buffer solution at pH 4 with various concentrations (0.045 and 0.09 % b/v), meanwhile tripolyphosphate (0.009 dan 0.018 % b/v) with volume compare (5 : 1). The characterizations of chitosan nanoparticles of extract were determined by measure its particle size, zeta potential, loading capacity, and determining efficiency value nanoparticle process formed. The results showed that the most stable extract were on concentration 0.68 and 0.9 mg/ml (kitosan 0.09 % b/v, TPP 0.018 % b/v) with 350 rpm stirring speed. The average of nanoparticle size were 190.9 and 162.87 nm. The zeta potential were 48.5 mV and 60.86 mV, the loading capacity were 2.96 and 5.33 %, than the loading efficiency were 35.75 and 45.26 %. Preparation of chitosan-extract of mahkota dewa fruits by ionic gelation method can produced nanoparticles and has a short range of size distribution, grade of uniformity and good stability. Key words: Mahkota dewa, nanoparticles, chitosan, ionic gelation.