The Application of Clinical Genetics (Jul 2022)

Deregulation of CircANXA2, Circ0075001, and CircFBXW7 Gene Expressions and Their Predictive Value in Egyptian Acute Myeloid Leukemia Patients

  • Tayel SI,
  • Soliman SE,
  • Ahmedy IA,
  • Abdelhafez M,
  • Elkholy AM,
  • Hegazy A,
  • Muharram NM

Journal volume & issue
Vol. Volume 15
pp. 69 – 85

Abstract

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Safaa I Tayel,1 Shimaa E Soliman,1 Iman A Ahmedy,2 Mohamed Abdelhafez,3 Aly M Elkholy,3 Amira Hegazy,4 Nashwa M Muharram1 1Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, 32511, Egypt; 2Clinical Pathology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, 32511, Egypt; 3Hematology Unit, Department of Internal Medicine, Faculty of Medicine, Menoufia University, Shebin El-Kom, 32511, Egypt; 4Clinical Oncology and Nuclear Medicine Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, 32511, EgyptCorrespondence: Safaa I Tayel, Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt, Email [email protected]: Acute myeloid leukemia (AML) is of heterogeneous pathogenesis and caused by alterations of multiple genes. CircRNAs act as oncogenes or tumor suppressors in numerous tumors and could be novel diagnostic and prognostic biomarkers. Few studies had incorporated circRNAs in AML.Aim of the Work: Assessment of circANXA2, circ0075001, and circFBXW7 gene expressions in AML patients. Evaluation of their relations with clinical, cytogenetic, and overall survival outcome to emphasize their diagnostic role and prognostic impact.Methods: This study was carried out on 120 subjects (66 AML patients and 54 controls). All subjects were subjected to gene expressions assay for circANXA2, circ0075001, circFBXW7 by quantitative real-time polymerase chain reaction.Results: Prominent overexpression of circANAX2 and circ0075001 in patients than control (P < 0.001), whereas circFBXW7 was markedly downregulated in patients than in control (P < 0.001). Moreover, circANXA2 with AUC 0.824, P < 0.001, had a sensitivity of 74.24%, specificity 88.89% whereas circ0075001 with AUC 0.855, P < 0.001, had the highest sensitivity of 83.33% and specificity 79.63%, and circFBXW7 with AUC 0.826, P < 0.001, had a sensitivity of 75.76% and specificity 74.07% in the distinction of AML patients from controls. Additionally, we find out that high expression of circANXA2 and circ0075001 correlated significantly with splenomegaly, hepatomegaly, less differentiated FAB subtypes (M5, M7), short overall survival, and had an adverse cytogenetic pattern.Conclusion: CircANXA2, circ0075001, and circFBXW7 gene expressions could serve as potential diagnostic biomarkers for AML disease. Moreover, CircANXA2 and circ0075001 exert poor prognostic effects on AML patients.Keywords: acute myeloid leukemia, CircRNAs, CircANXA2, circ0075001, circFBXW7

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