1,2-3H-Cholesterol as a tracer in studies of human cholesterol metabolism

Abstract

1,2-3H-Cholesterol was fed to a subject together with 4-14C-cholesterol at a known 3H/14C ratio. The ratio was satisfactorily preserved in cholesterol recovered from plasma, red cells, and bile, and in bile acids of bile.Isotopic fractionation was seen during thin-layer chromatographic isolation of cholesterol. In work with 1,2-3H-cholesterol or its metabolites care should be taken to recover chromatographic bands in their entirety.

Keywords

• cholesterol
• 1,2-3H-cholesterol
• 4-14C-cholesterol
• thin-layer chromatography
• isotope effect
• plasma