Frontiers in Genetics (2018-07-01)

CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities

  • Bhaskar Roy,
  • Jing Zhao,
  • Jing Zhao,
  • Chao Yang,
  • Wen Luo,
  • Teng Xiong,
  • Yong Li,
  • Xiaodong Fang,
  • Guanjun Gao,
  • Chabungbam O. Singh,
  • Lise Madsen,
  • Lise Madsen,
  • Lise Madsen,
  • Yong Zhou,
  • Karsten Kristiansen,
  • Karsten Kristiansen

Journal volume & issue
Vol. 9


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Clustered Regularly Interspaced Palindromic Repeats (CRISPR) and Cascade 9 (also known as Cas9, CRISPR associated protein 9) confer protection against invading viruses or plasmids. The CRISPR/Cascade 9 system constitutes one of the most powerful genome technologies available to researchers today. So far, this technology has enabled efficient genome editing and modification in several model organisms and has successfully been used in biomedicine and biomedical engineering. However, challenges for efficient and safe genetic manipulation in several organisms persist. Here, we review functional approaches and future challenges associated with the use of the CRISPR/Cascade 9 genome editing system and discuss opportunities, ethical issues and future directions within this field.