Metabarcoding and Metagenomics (Feb 2021)
Metadata standards and practical guidelines for specimen and DNA curation when building barcode reference libraries for aquatic life
- Frédéric Rimet,
- Eva Aylagas,
- Angel Borja,
- Agnès Bouchez,
- Alexis Canino,
- Christian Chauvin,
- Teofana Chonova,
- Fedor Ciampor Jr,
- Filipe О. Costa,
- Benoit J. D. Ferrari,
- Romain Gastineau,
- Chloé Goulon,
- Muriel Gugger,
- Maria Holzmann,
- Regine Jahn,
- Maria Kahlert,
- Wolf-Henning Kusber,
- Christophe Laplace-Treyture,
- Florian Leese,
- Frederik Leliaert,
- David G. Mann,
- Frédéric Marchand,
- Vona Méléder,
- Jan Pawlowski,
- Serena Rasconi,
- Sinziana Rivera,
- Rodolphe Rougerie,
- Magali Schweizer,
- Rosa Trobajo,
- Valentin Vasselon,
- Régis Vivien,
- Alexander Weigand,
- Andrzej Witkowski,
- Jonas Zimmermann,
- Torbjørn Ekrem
Affiliations
- Frédéric Rimet
- INRAE
- Eva Aylagas
- King Abdullah University of Science and Technology
- Angel Borja
- AZTI
- Agnès Bouchez
- INRAE
- Alexis Canino
- INRAE
- Christian Chauvin
- INRAE
- Teofana Chonova
- INRAE
- Fedor Ciampor Jr
- Slovak Academy of Sciences
- Filipe О. Costa
- University of Minho
- Benoit J. D. Ferrari
- Swiss Centre for Applied Ecotoxicology
- Romain Gastineau
- University of Szczecin
- Chloé Goulon
- INRAE
- Muriel Gugger
- Institut Pasteur
- Maria Holzmann
- University of Geneva
- Regine Jahn
- Freie Universität Berlin
- Maria Kahlert
- Swedish University of Agricultural Sciences
- Wolf-Henning Kusber
- Freie Universität Berlin
- Christophe Laplace-Treyture
- INRAE
- Florian Leese
- University of Duisburg-Essen
- Frederik Leliaert
- Meise Botanic Garden
- David G. Mann
- Royal Botanic Garden Edinburgh
- Frédéric Marchand
- INRAE
- Vona Méléder
- University of Nantes
- Jan Pawlowski
- Institute of Oceanology, Polish Academy of Sciences
- Serena Rasconi
- INRAE
- Sinziana Rivera
- INRAE
- Rodolphe Rougerie
- Sorbonne Université
- Magali Schweizer
- University of Angers
- Rosa Trobajo
- Institute for Food and Agricultural Research and Technology
- Valentin Vasselon
- SCIMABIO Interface
- Régis Vivien
- Swiss Centre for Applied Ecotoxicology
- Alexander Weigand
- Musée national d’histoire naturelle de Luxembourg
- Andrzej Witkowski
- University of Szczecin
- Jonas Zimmermann
- Freie Universität Berlin
- Torbjørn Ekrem
- Norwegian University of Science and Technology
- DOI
- https://doi.org/10.3897/mbmg.5.58056
- Journal volume & issue
-
Vol. 5
pp. 17 – 33
Abstract
Read online Read online Read online
DNA barcoding and metabarcoding is increasingly used to effectively and precisely assess and monitor biodiversity in aquatic ecosystems. As these methods rely on data availability and quality of barcode reference libraries, it is important to develop and follow best practices to ensure optimal quality and traceability of the metadata associated with the reference barcodes used for identification. Sufficient metadata, as well as vouchers, corresponding to each reference barcode must be available to ensure reliable barcode library curation and, thereby, provide trustworthy baselines for downstream molecular species identification. This document (1) specifies the data and metadata required to ensure the relevance, the accessibility and traceability of DNA barcodes and (2) specifies the recommendations for DNA harvesting and for the storage of both voucher specimens/samples and barcode data.
