Nature Communications (Feb 2017)
A substrate-bound structure of cyanobacterial biliverdin reductase identifies stacked substrates as critical for activity
- Haruna Takao,
- Kei Hirabayashi,
- Yuki Nishigaya,
- Haruna Kouriki,
- Tetsuko Nakaniwa,
- Yoshinori Hagiwara,
- Jiro Harada,
- Hideaki Sato,
- Toshimasa Yamazaki,
- Yoichi Sakakibara,
- Masahito Suiko,
- Yujiro Asada,
- Yasuhiro Takahashi,
- Ken Yamamoto,
- Keiichi Fukuyama,
- Masakazu Sugishima,
- Kei Wada
Affiliations
- Haruna Takao
- Organization for Promotion of Tenure Track, University of Miyazaki
- Kei Hirabayashi
- Organization for Promotion of Tenure Track, University of Miyazaki
- Yuki Nishigaya
- Advanced Analysis Center, National Agriculture and Food Research Organization
- Haruna Kouriki
- Organization for Promotion of Tenure Track, University of Miyazaki
- Tetsuko Nakaniwa
- Department of Biological Sciences, Graduate School of Science, Osaka University
- Yoshinori Hagiwara
- Department of Biochemistry and Applied Chemistry, National Institute of Technology, Kurume College
- Jiro Harada
- Department of Medical Biochemistry, Kurume University School of Medicine
- Hideaki Sato
- Department of Medical Biochemistry, Kurume University School of Medicine
- Toshimasa Yamazaki
- Advanced Analysis Center, National Agriculture and Food Research Organization
- Yoichi Sakakibara
- Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
- Masahito Suiko
- Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
- Yujiro Asada
- Department of Pathology, Faculty of Medicine, University of Miyazaki
- Yasuhiro Takahashi
- Division of Life Science, Graduate School of Science and Engineering, Saitama University
- Ken Yamamoto
- Department of Medical Biochemistry, Kurume University School of Medicine
- Keiichi Fukuyama
- Department of Biological Sciences, Graduate School of Science, Osaka University
- Masakazu Sugishima
- Department of Medical Biochemistry, Kurume University School of Medicine
- Kei Wada
- Organization for Promotion of Tenure Track, University of Miyazaki
- DOI
- https://doi.org/10.1038/ncomms14397
- Journal volume & issue
-
Vol. 8,
no. 1
pp. 1 – 10
Abstract
Biliverdin reductase (BVR) catalyses the last step in haem degradation. Here the authors present the crystal structure of cyanobacterial BVR bound to its substrate biliverdin and oxidised cofactor NADP+, which was used to propose the catalytic mechanism of this enzyme.
