Shanghai Jiaotong Daxue xuebao. Yixue ban (Mar 2023)

Expression of tetraspanin 1 in breast cancer and its mechanism in promoting the progression of breast cancer

  • CAO Yuan,
  • WANG Hongxia,
  • ZHU Ying,
  • LI Junjian

DOI
https://doi.org/10.3969/j.issn.1674-8115.2023.03.004
Journal volume & issue
Vol. 43, no. 3
pp. 293 – 300

Abstract

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Objective·To investigate the expression of tetraspanin 1 (TSPAN1) in breast cancer and its effect on the migration and invasion of breast cancer cells.Methods·Immunohistochemistry staining (IHC) was used to detect the expression of TSPAN1 in breast cancer tissues and para-tumor tissues from 106 clinical patients, and to analyze its correlation with the clinical characteristics of patients. The Cancer Genome Atlas (TCGA) database was used to analyze the expression of TSPAN1 mRNA in breast cancer tissues and para-tumor tissues, and its correlation with the clinical characteristics of patients. After down-regulation of TSPAN1 in breast cancer cells MDA-MB-231 and SUM159PT, the effects of TSPAN1 on migration and invasion of cells were detected by wound healing assay and transwell assay, and the expression of epithelial-mesenchymal transition (EMT)-related proteins (E-cadherin, N-cadherin and vimentin) were detected by Western blotting.Results·IHC results showed that the expression of TSPAN1 in breast cancer tissues was higher than that in para-tumor tissues (P=0.000), and the expression of TSPAN1 in total TNM stages, M stages, N stages and pathological grades of breast cancer tissues showed statistically significant differences (all P<0.05). The results of transcriptome sequencing data from TCGA database showed that the expression of TSPAN1 mRNA in breast cancer tissues was higher than that in para-tumor tissues (P=0.000), and its expression in TNM Ⅲ‒Ⅳ stages was higher than that in TNMⅠ‒Ⅱ stages (P=0.007). After down-regulation of TSPAN1, the migration and invasion ability of MDA-MB-231 and SUM159PT cells were decreased, the expression of E-cadherin was increased and the expressions of N-cadherin and vimentin were decreased (all P<0.05).Conclusion·TSPAN1 is highly expressed in breast cancer tissues, which can promote the migration and invasion of breast cancer cells.

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