Microsomal activation, and SH-SY5Y cell toxicity studies of tremetone and 6-hydroxytremetone isolated from rayless goldenrod (Isocoma pluriflora) and white snakeroot (Agertina altissima), respectively
Benedict T. Green,
Stephen T. Lee,
T. Zane Davis,
Kevin D. Welch
Affiliations
Benedict T. Green
Corresponding author. Poisonous Plant Research Laboratory, ARS/USDA, 1150 East 1400 North, Logan, Utah, 84341, USA.; Poisonous Plant Research Laboratory, Agricultural Research Service, United States Department of Agriculture, Logan, Utah, 84341, USA
Stephen T. Lee
Poisonous Plant Research Laboratory, Agricultural Research Service, United States Department of Agriculture, Logan, Utah, 84341, USA
T. Zane Davis
Poisonous Plant Research Laboratory, Agricultural Research Service, United States Department of Agriculture, Logan, Utah, 84341, USA
Kevin D. Welch
Poisonous Plant Research Laboratory, Agricultural Research Service, United States Department of Agriculture, Logan, Utah, 84341, USA
This research compared the cytotoxic actions of the benzofuran ketone, tremetone in B16 murine melanoma cells to SH-SY5Y human neuroblastoma cells with an MTT assay. Tremetone was not cytotoxic in B16 cells. In SH-SY5Y cells, concentration-dependent tremetone cytotoxicity occurred without microsomal activation. No cytotoxicity was observed with 6-hydroxytremetone. This suggests that SH-SY5Y cells are a better model for the cytotoxic actions of tremetone and that tremetone is toxic without microsomal activation. Keywords: Tremetone, White snakeroot, Ageratina altissima, Rayless goldenrod, Eupatorium rugosum
