DETERMINAÇÃO E DISTRIBUIÇÃO DE Cu, Fe E Zn EM CAMAPU POR ICP-OES

Abstract

This study aimed to determine the total contents of copper, iron, and zinc in camapu and protein fractions by inductively coupled plasma emission spectrometry. Firstly, lipid removal was performed using chloroform and methanol (2:1). Protein fractions were obtained after sequential extraction using four extractors (water, 0.5 mol L-1 sodium chloride, 50% acetic acid v v-1 and 0.05 mol L-1 sodium hydroxide). The camapu sample and protein fractions were digested in a cavity-microwave oven with diluted nitric acid and hydrogen peroxide. The accuracy of the procedure was evaluated using the addition and recovery method in the digests and protein extracts. All the elements were determined in the protein fractions. Levels of Cu, Fe, and Zn ranged from 0.07 to 0.5 mg kg-1 were found in the lipid fraction. The residue presented higher iron content compared to other elements. Higher Cu and Zn contents were found associated with albumin. All elements studied were found in the protein fractions, and the total protein concentration determined in each protein fraction was 5.74 mg kg-1 (albumins), 0.28 mg kg-1 (globulins), 4.46 mg kg-1 (glutelins 1) and 0.46 mg kg-1 (glutelins 2).