Generation of three clones (JUCGRMi002-A, B, C) of induced pluripotent stem cells from a Parkinson’s disease patient with SNCA duplication

Kei-ichi Ishikawa; Takahiro Shiga; Hiroyo Yoshino; Kenya Nishioka; Nobutaka Hattori; Wado Akamatsu

Stem Cell Research (Feb 2024)

Generation of three clones (JUCGRMi002-A, B, C) of induced pluripotent stem cells from a Parkinson’s disease patient with SNCA duplication

Kei-ichi Ishikawa,
Takahiro Shiga,
Hiroyo Yoshino,
Kenya Nishioka,
Nobutaka Hattori,
Wado Akamatsu

Affiliations

Kei-ichi Ishikawa: Center for Genomic and Regenerative Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan; Department of Neurology, Juntendo University School of Medicine, Tokyo, Japan; Department of Research and Development for Organoids, Juntendo University School of Medicine, Tokyo, Japan; Corresponding author.
Takahiro Shiga: Center for Genomic and Regenerative Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan
Hiroyo Yoshino: Research Institute for Diseases of Old Age, Juntendo University Graduate School of Medicine, Tokyo, Japan
Kenya Nishioka: Department of Neurology, Juntendo Tokyo Koto Geriatric Medical Center, Tokyo, Japan
Nobutaka Hattori: Center for Genomic and Regenerative Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan; Department of Neurology, Juntendo University School of Medicine, Tokyo, Japan; Department of Research and Development for Organoids, Juntendo University School of Medicine, Tokyo, Japan; Neurodegenerative Disorders Collaborative Laboratory, RIKEN Center for Brain Science, Saitama, Japan
Wado Akamatsu: Center for Genomic and Regenerative Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan

Journal volume & issue: Vol. 74
p. 103296

Abstract

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Parkinson’s disease is the second most common neurodegenerative disorder and is pathologically characterized by synuclein-rich aggregations (Lewy bodies) in neurons. Multiplication of the synuclein gene (SNCA) increases the mRNA and protein levels of synuclein, resulting in autosomal dominant hereditary Parkinson’s disease. In the present study, we established three isogenic induced pluripotent stem cells (iPSCs) from a patient harboring SNCA duplication, which showed pluripotency, three-germ layer differentiation capacity, and normal karyotypes.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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