Pharmacogenomics and Personalized Medicine (Jun 2022)

Inducible Nitric Oxide Synthase iNOS-954-G>C and Ex16+14-C>T Gene Polymorphisms and Susceptibility to Vitiligo in the Saudi Population

  • Al-Harthi F,
  • Huraib GB,
  • Mustafa M,
  • Al-Qubaisy Y,
  • Al-Nomair N,
  • Abdurrahman N,
  • Al-Asmari A

Journal volume & issue
Vol. Volume 15
pp. 603 – 612

Abstract

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Fahad Al-Harthi,1 Ghaleb Bin Huraib,2 Md Mustafa,2 Yasser Al-Qubaisy,1 Naif Al-Nomair,1 Nour Abdurrahman,1 Abdulrahman Al-Asmari2 1Deparment of Dermatology, Prince Sultan Military Medical City, Riyadh, Saudi Arabia; 2Scientific Research Center, Health service directorate, Riyadh, Saudi ArabiaCorrespondence: Abdulrahman Al-Asmari, Scientific Research Center, Health service directorate, P.O. Box 8524, Riyadh, 11492, Saudi Arabia, Email [email protected]: Vitiligo is an acquired pigmentary skin disorder with regional disappearance of melanocytes. Multigenic inheritance has been proposed in the pathogenesis of vitiligo. The present study aimed to investigate the possible association of inducible nitric oxide synthase polymorphisms iNOS-954-G/C (rs1800482 G>C) and iNOS-Ex16+14-C/T (rs2297518 C>T) with vitiligo in the Saudi population, if any.Methods: We included 120 vitiligo cases and an equal number of age matched healthy controls. Polymerase chain reaction with restriction fragment length polymorphism method was used for the analysis of genetic polymorphisms.Results: The heterozygous (GC), (GC + CC) combined genotype and variant allele; C allele of rs1800482 G>C were associated significantly (p C was co-inherited with common genotype (CC) and heterozygous genotype (CT) of rs2297518 C>T, the risk of vitiligo was significantly increased ((OR = 4.51, 95% CI = 2.18– 9.33, p = 0.001) and (OR = 3.60, 95% CI = 1.61– 8.01, p = 0.001)) respectively. None of the rs1800482 G>C and rs2297518 C>T genotypes and alleles have been associated with non-segmental vitiligo in terms of gender, age of onset, and types of vitiligo.Conclusion: The heterozygous (GC), (GC+CC) combined genotype and variants allele; C allele of rs1800482 G>C, may cause overproduction of NO, which has been linked to melanocyte loss by increasing oxidative stress and decreasing melanocyte adhesion to the extracellular matrix components, and thus could be an associative risk factor for vitiligo.Keywords: vitiligo, non-segmental, genetic polymorphism, rs1800482 G>C, rs2297518 C>T, nitric oxide, nitric oxide synthase

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