Genetic programming of macrophages generates an in vitro model for the human erythroid island niche

Martha Lopez-Yrigoyen; Cheng-Tao Yang; Antonella Fidanza; Luca Cassetta; A. Helen Taylor; Angela McCahill; Erica Sellink; Marieke von Lindern; Emile van den Akker; Joanne C. Mountford; Jeffrey W. Pollard; Lesley M. Forrester

doi:10.1038/s41467-019-08705-0

Nature Communications (Feb 2019)

Genetic programming of macrophages generates an in vitro model for the human erythroid island niche

Martha Lopez-Yrigoyen,
Cheng-Tao Yang,
Antonella Fidanza,
Luca Cassetta,
A. Helen Taylor,
Angela McCahill,
Erica Sellink,
Marieke von Lindern,
Emile van den Akker,
Joanne C. Mountford,
Jeffrey W. Pollard,
Lesley M. Forrester

Affiliations

Martha Lopez-Yrigoyen: MRC Centre for Regenerative Medicine, University of Edinburgh
Cheng-Tao Yang: MRC Centre for Regenerative Medicine, University of Edinburgh
Antonella Fidanza: MRC Centre for Regenerative Medicine, University of Edinburgh
Luca Cassetta: MRC Centre for Reproductive Health, University of Edinburgh, Queens Medical Research Institute
A. Helen Taylor: MRC Centre for Regenerative Medicine, University of Edinburgh
Angela McCahill: Scottish National Blood Transfusion Service, The Jack Copland Centre
Erica Sellink: Sanquin Research, Department of Hematopoiesis, The Netherlands and Landsteiner Laboratory
Marieke von Lindern: Sanquin Research, Department of Hematopoiesis, The Netherlands and Landsteiner Laboratory
Emile van den Akker: Sanquin Research, Department of Hematopoiesis, The Netherlands and Landsteiner Laboratory
Joanne C. Mountford: Scottish National Blood Transfusion Service, The Jack Copland Centre
Jeffrey W. Pollard: MRC Centre for Reproductive Health, University of Edinburgh, Queens Medical Research Institute
Lesley M. Forrester: MRC Centre for Regenerative Medicine, University of Edinburgh

DOI: https://doi.org/10.1038/s41467-019-08705-0
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 11

Abstract

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In vitro differentiation of red blood cells (RBCs) is a desirable therapy for various disorders. Here the authors develop a culture system using stem cell-derived macrophages to show that inducible expression of a transcription factor, KLF1, enhances RBC production, potentially through the induction of three soluble factors, ANGPTL7, IL33 and SERPINB2.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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