Drug Design, Development and Therapy (Mar 2022)
Salidroside Protects Acute Kidney Injury in Septic Rats by Inhibiting Inflammation and Apoptosis
Abstract
Heng Fan,1,* Bin-jie Su,2,* Jian-wei Le,1 Jian-hua Zhu1 1Department of Intensive Care Unit, Ningbo First Hospital, Ningbo, Zhejiang Province, People’s Republic of China; 2Department of Intensive Care Unit, Ningbo First Hospital Haishu Branch, Ningbo, Zhejiang Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jian-hua Zhu, Department of Intensive Care Unit, Ningbo First Hospital, No.59 Liuting Road, Ningbo, Zhejiang Province, 315010, People’s Republic of China, Tel/Fax +86-574-87084625, Email [email protected]: To clarify the protective effect and mechanism of salidroside (SLDS) on acute kidney injury (AKI) in septic rats.Methods: We pretreated rats with different doses of SLDS and analyzed the impact of SLDS on the survival of septic rats. We evaluated the levels of inflammatory factors in rats, the expression of NF-ƙB p65 in the kidney, and the apoptosis of kidney tubular epithelial cells (KTECs).Results: SLDS significantly decreased the mortality of septic rats, and it reduced the levels of TNF-α, IL-1β, and IL-17A in plasma and kidneys and decreased the levels of serum creatinine, plasma renal injury molecule-1 and plasma neutrophil gelatin-associated lipocalin. Moreover, SLDS could significantly decrease the expression of NF-ƙB p65 in kidney tissues and the apoptotic number of KETCs, while reducing the mRNA levels of Caspase-3 and Bax mRNA, and increasing the level of Bcl-2 mRNA.Conclusion: SLDS pretreatment protects against AKI in septic rats by inhibiting the inflammation of kidney and the apoptosis of KTECs.Keywords: salidroside, acute kidney injury, sepsis, apoptosis, inflammation
