Protocol for embedded 3D printing of heart tissues using thiol-norbornene collagen

Zili Gao; Shenglong Ding; Tingting Fan; Wenhui Huang; Xiyuan Zhao; Xin Liu; Wenli Liu; Mingzhu Zhang; Qi Gu

STAR Protocols (Jun 2024)

Protocol for embedded 3D printing of heart tissues using thiol-norbornene collagen

Zili Gao,
Shenglong Ding,
Tingting Fan,
Wenhui Huang,
Xiyuan Zhao,
Xin Liu,
Wenli Liu,
Mingzhu Zhang,
Qi Gu

Affiliations

Zili Gao: Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, P.R. China
Shenglong Ding: Department of Foot and Ankle Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China
Tingting Fan: Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, P.R. China
Wenhui Huang: Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, P.R. China
Xiyuan Zhao: Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, P.R. China
Xin Liu: Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, P.R. China; Key Laboratory of Organ Regeneration and Transplantation of the Ministry of Education, Jilin University, Changchun 130061, P.R. China
Wenli Liu: Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, P.R. China
Mingzhu Zhang: Department of Foot and Ankle Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China; Corresponding author
Qi Gu: Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, P.R. China; Corresponding author

Journal volume & issue: Vol. 5, no. 2
p. 102994

Abstract

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Summary: Here, we present a protocol for 3D printing heart tissues using thiol-norbornene photoclick collagen (NorCol). We describe steps for synthesizing NorCol, preparing bioink and the support bath, and cell-laden printing. We then detail procedures for the loading of C2C12 cells into NorCol, ensuring structural integrity and cell viability after printing. This protocol is adaptable to various cell lines and allows for the printing of diverse complex structures, which can be used in drug screening and disease modeling. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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