Degradation of azo dye by bacterium, Alishewanella sp. CBL-2 isolated from industrial effluent and its potential use in decontamination of wastewater

Abstract

In the present study, Alishewanella sp. CBL-2 was characterized on the basis of biochemical and 16S rRNA sequencing. The bacterium was found to decolorize 83% Sumifex Tourqi blue within a period of 6 days at 37 °C and pH 7. The predominant form of the enzyme was found to be extracellular (78%) as compared to intracellular (16%). The breakdown of azo bond was confirmed through thin layer chromatography (TLC) analysis as new bands and peaks were observed in chromatograms of extracted metabolites of decolorized samples. Growth of beneficial microbial fauna in the presence of bacterial decolorized wastewater indicates that this wastewater is safe for the survival of microorganisms. High bioremediation potential makes Alishewanella sp. CBL-2 an impending foundation for green chemistry to remove azo dyes from industrial wastewater.

Keywords

• Alishewanella sp. CBL-2
• azo dye
• decolorization
• TLC