European Journal of Biological Research (Feb 2023)

Antidiabetic potential of mucilage fraction extracted from Astragalus gyzensis seeds

  • Aicha Tedjani,
  • Zakaria Boual,
  • Mohamed Didi Ould El Hadj,
  • Touhami Lanez,
  • Hakim Belkhalfa,
  • Zainab El Alaoui-Talibi,
  • Cherkaoui El Modafar,
  • Slim Abdelkafi,
  • Imen Fendri,
  • Didier Le Cerf,
  • Pascal Dubessay,
  • Cédric Delattre,
  • Pierre Guillaume,
  • Philippe Michaud

Journal volume & issue
Vol. 13, no. 1

Abstract

Read online

The objective of the current work is to extract a new mucilage fraction from Astragalus gyzensis Bunge. seeds, which are collected from the El-Oued province (septentrional Algerian Sahara) and evaluated for their antidiabetic potential. The mucilage fraction is obtained using hot water extraction followed by alcoholic precipitation of polysaccharides by cold ethanol (96%). The primary investigation was performed by describing the main structural features of the extract through colorimetric assays, Fourier-transform infrared spectroscopy and thin-layer chromatography analysis using two systems. Biological activity was also monitored by antidiabetic activity by testing the inhibition of α-amylase and α-glucosidase enzymes in vitro. The extraction yield was 20.69%. The chemical composition mainly consisted of 78.60±0.29% carbohydrates, among them 63.92±0.67% neutral sugar, 15.78±0.76% uronic acid, 8.08±0.04% proteins and 2.57±0.05% phenolic compounds. The results obtained by thin-layer chromatography analysis showed the dominance of mannose and galactose. Fourier-transform infrared spectrum showed characteristic bands expected galactomannans. The investigations highlighted the antihyperglycemic effect in a dose-dependent manner by the inhibition of the α-amylase enzyme (IC50=0.8±0.005 mg/mL). These factors make it suitable for the industrial application of dietary supplement fiber made for diabetic individuals. DOI: http://dx.doi.org/10.5281/zenodo.7618530

Keywords