In-vitro phytopharmacological and anticancer activity of Loranthus Longiflorus Desv. Var. Falcatuskurz against the human lung cancer cells

Abstract

In this study, we evaluated anticancer activity of methanol extract and purified compound from Loranthus Longiflorus against Human lung cancer cells (A-549), human cervical carcinoma (HeLa) and human hepatocellular carcinoma cell line (HepG2). Cytotoxicity of Loranthus Longiflorus was investigated using MTT assay. Doxorubicin was used as the positive control. MTT assay showed activity against all three tested cell lines. The cytotoxicity activities expressed as percentage of cell viability and the compound was effective on HepG2, HeLa and A-549 cell lines. Cytotoxicity of methanolic extract of LLF against A-549 cell line was measured and the IC50 value of methanol extract of LLF was 17 ± 1.5 µg/ml. In HeLa cell line, the IC50 value of methanol extract of LLF was16 ± 1.0 µg/ml. Cytotoxicity of methanolic extract of LLF against HepG2 cell line was also evaluated. IC50 value of Doxorubicin (standard) was 11 ± 1.0 µg/ml and IC50 value of methanol extract of LLF was 18 ± 0.5 µg/ml. Similarly cytotoxicity showed a significant activity of the compound LLF on HepG2, HeLa and A-549 cell lines. The IC50 values of compound were 13.5 ± 1.5 µg/ml, 15 ± 1.5 µg/ml, 17 ± 0.5 µg/ml against A-549, HeLa and HepG2 cell lines. The present findings confirmed cytotoxic effect of methanol extract and purified compound from Loranthus Longiflorus against A-549, HeLa and HepG2 cell lines. Keywords: Medicinal plants, Herb, Anticancer, Cell lines, Cytotoxicity