Proteome-wide identification of the endogenous ADP-ribosylome of mammalian cells and tissue

Rita Martello; Mario Leutert; Stephanie Jungmichel; Vera Bilan; Sara C. Larsen; Clifford Young; Michael O. Hottiger; Michael L. Nielsen

doi:10.1038/ncomms12917

Nature Communications (Sep 2016)

Proteome-wide identification of the endogenous ADP-ribosylome of mammalian cells and tissue

Rita Martello,
Mario Leutert,
Stephanie Jungmichel,
Vera Bilan,
Sara C. Larsen,
Clifford Young,
Michael O. Hottiger,
Michael L. Nielsen

Affiliations

Rita Martello: Department of Proteomics, Faculty of Health Sciences, The Novo Nordisk Foundation Centre for Protein Research, University of Copenhagen
Mario Leutert: Department of Molecular Mechanisms of Disease, University of Zurich
Stephanie Jungmichel: Department of Proteomics, Faculty of Health Sciences, The Novo Nordisk Foundation Centre for Protein Research, University of Copenhagen
Vera Bilan: Department of Molecular Mechanisms of Disease, University of Zurich
Sara C. Larsen: Department of Proteomics, Faculty of Health Sciences, The Novo Nordisk Foundation Centre for Protein Research, University of Copenhagen
Clifford Young: Department of Proteomics, Faculty of Health Sciences, The Novo Nordisk Foundation Centre for Protein Research, University of Copenhagen
Michael O. Hottiger: Department of Molecular Mechanisms of Disease, University of Zurich
Michael L. Nielsen: Department of Proteomics, Faculty of Health Sciences, The Novo Nordisk Foundation Centre for Protein Research, University of Copenhagen

DOI: https://doi.org/10.1038/ncomms12917
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 13

Abstract

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ADP-ribosylation is a reversible post-translational protein modification involved in many cellular processes. Here the authors describe a sensitive approach for the analysis of ADP-ribosylation sites under physiologic conditions and identify lysine residues as in vivotargets of ADP-ribosylation.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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