Egyptian Journal of Chest Disease and Tuberculosis (Apr 2013)

Resazurin Microtiter Assay Plate method for detection of susceptibility of multidrug resistant Mycobacterium tuberculosis to second-line anti-tuberculous drugs

  • Reham A. Khalifa,
  • Marwa S. Nasser,
  • Ashraf A. Gomaa,
  • Nehad M. Osman,
  • Hala M. Salem

DOI
https://doi.org/10.1016/j.ejcdt.2013.05.008
Journal volume & issue
Vol. 62, no. 2
pp. 241 – 247

Abstract

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Background: Many developed countries need a diagnostic test that is accurate, rapid, and economical in detecting multidrug-resistant tuberculosis. This study aims to evaluate Resazurin Microtiter Assay Plate in detecting susceptibilities of multidrug resistant Mycobacterium tuberculosis to second line anti tuberculous drugs. Methods: Susceptibility of multidrug resistant M. tuberculosis was evaluated against 5 second line anti tuberculous drugs by the colorimetric method using the Resazurin Microtiter Assay Plate Testing (REMA). Results: Drug susceptibility testing for the second-line drugs was performed for 34 MDR M. tuberculosis isolates isolated from 40 sputum samples. Results of REMA were available after 8 days of incubation. The agreement between the two tests for para-aminosalicylic acid, ethionamide, ofloxacin, kanamycin monosulfate and cycloserine was found to be 97.05%, 94.11%, 97.05%, 97.05% and 100%, respectively. The efficacy of REMA plate assay for PAS, ETH, OFX, KAN and CYL was 97.05%, 94.11%, 97.05%, 97.05% and 100%, respectively. Conclusion: Resazurin test was found to be reliable, simple to perform for the rapid detection of anti-tuberculous drug resistance and economically inexpensive.

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