Nature Communications (Sep 2019)

Massively parallel RNA device engineering in mammalian cells with RNA-Seq

  • Joy S. Xiang,
  • Matias Kaplan,
  • Peter Dykstra,
  • Michaela Hinks,
  • Maureen McKeague,
  • Christina D. Smolke

DOI
https://doi.org/10.1038/s41467-019-12334-y
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 16

Abstract

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Synthetic RNA-based devices can dynamically control a wide range of processes. Here the authors develop a quantitative and high-throughput mammalian cell-based RNA-seq assay to efficiently engineer ribozyme switches.