Visualizing Dynamic Performance of Lipid Droplets in a Parkinson’s Disease Model via a Smart Photostable Aggregation-Induced Emission Probe
Lihua Li,
Fan Zhou,
Qun Gao,
Yao Lu,
Xingyi Xu,
Rong Hu,
Zhiming Wang,
Mingying Peng,
Zhongmin Yang,
Ben Zhong Tang
Affiliations
Lihua Li
State Key Laboratory of Luminescent Materials and Devices, Applied Techniques School of Materials Science and Engineering and Center for Aggregation-Induced Emission, Guangdong Provincial Key Laboratory of Fiber Laser Materials, School of Materials Science and Engineering, School of Physics, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China
Fan Zhou
State Key Laboratory of Luminescent Materials and Devices, Applied Techniques School of Materials Science and Engineering and Center for Aggregation-Induced Emission, Guangdong Provincial Key Laboratory of Fiber Laser Materials, School of Materials Science and Engineering, School of Physics, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China
Qun Gao
Department of Neurosurgery, Beijing Hospital, National Center of Gerontology, Graduate School of Peking Union Medical College, Beijing 100005, China
Yao Lu
Department of Orthopedics, Zhujiang Hospital, Southern Medical University, 253 Gongye Road, Guangzhou 510282, China
Xingyi Xu
State Key Laboratory of Luminescent Materials and Devices, Applied Techniques School of Materials Science and Engineering and Center for Aggregation-Induced Emission, Guangdong Provincial Key Laboratory of Fiber Laser Materials, School of Materials Science and Engineering, School of Physics, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China
Rong Hu
State Key Laboratory of Luminescent Materials and Devices, Applied Techniques School of Materials Science and Engineering and Center for Aggregation-Induced Emission, Guangdong Provincial Key Laboratory of Fiber Laser Materials, School of Materials Science and Engineering, School of Physics, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China
Zhiming Wang
State Key Laboratory of Luminescent Materials and Devices, Applied Techniques School of Materials Science and Engineering and Center for Aggregation-Induced Emission, Guangdong Provincial Key Laboratory of Fiber Laser Materials, School of Materials Science and Engineering, School of Physics, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China; Corresponding author
Mingying Peng
State Key Laboratory of Luminescent Materials and Devices, Applied Techniques School of Materials Science and Engineering and Center for Aggregation-Induced Emission, Guangdong Provincial Key Laboratory of Fiber Laser Materials, School of Materials Science and Engineering, School of Physics, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China; Corresponding author
Zhongmin Yang
State Key Laboratory of Luminescent Materials and Devices, Applied Techniques School of Materials Science and Engineering and Center for Aggregation-Induced Emission, Guangdong Provincial Key Laboratory of Fiber Laser Materials, School of Materials Science and Engineering, School of Physics, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China; Corresponding author
Ben Zhong Tang
State Key Laboratory of Luminescent Materials and Devices, Applied Techniques School of Materials Science and Engineering and Center for Aggregation-Induced Emission, Guangdong Provincial Key Laboratory of Fiber Laser Materials, School of Materials Science and Engineering, School of Physics, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China; Department of Chemistry and Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong, China; Corresponding author
Summary: Parkinson’s disease (PD) is a complex neurodegenerative disease affected by diverse factors, and lipid droplets (LDs) are increasingly recognized as major players in PD because of their relevance to neuron activity. However, long-term dynamic changes of LDs and their relative activity remain unclear. Here, an aggregation-induced emission (AIE) probe named 2-DPAN was prepared and employed to visualize dynamic processes of LDs in a 6-hydroxydopamine model of PD for the first time, and LDs' accumulation-peak/plateau-decrease were confirmed. We further found a close relationship between LDs and variation in mitochondrial activity. Strikingly, the progression of cell death was accelerated by lipase, whereas pre-stimulation of LDs by unsaturated fatty acid-oleic acid decreased the death process by inhibiting excessive reactive oxygen species (ROS) and fatty acid production, thereby protecting mitochondria. The utilization of 2-DPAN demonstrates the importance of LDs in neuronal homeostasis, and effective tuning of LDs may prevent or inhibit PD progression. : Disease; Chemical Synthesis; Materials Design Subject Areas: Disease, Chemical Synthesis, Materials Design
