Stem Cell Research (Apr 2017)

Establishment of MUi009 – A human induced pluripotent stem cells from a 32 year old male with homozygous β°-thalassemia coinherited with heterozygous α-thalassemia 2

  • Wasinee Wongkummool,
  • Warun Maneepitasut,
  • Pirut Tong-ngam,
  • Amornrat Tangprasittipap,
  • Thongperm Munkongdee,
  • Chanikarn Boonchuay,
  • Saovaros Svasti,
  • Narisorn Kitiyanant,
  • Kittiphong Paiboonsukwong,
  • Suthat Fucharoen,
  • Alisa Tubsuwan

DOI
https://doi.org/10.1016/j.scr.2017.02.012
Journal volume & issue
Vol. 20, no. C
pp. 80 – 83

Abstract

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The thalassemias are a group of genetic disorders characterized by a deficiency in the synthesis of globin chains. In this study the MUi009-A human induced pluripotent stem cell line was successfully generated from peripheral blood CD34+ haematopoietic progenitors of a 32 year old male who had coinherited a homozygous β°-thalassemia mutation at codon 41/42 (-TCTT) and a heterozygous α-thalassemia 4.2 deletion. The MUi009-A cell line exhibited embryonic stem cell characteristics with consistent pluripotency marker expression and the capability of differentiating into the three germ layers. The cell line may provide a tool for drug testing and gene therapy studies.