Nature Communications (Jun 2017)
Ezrin enhances line tension along transcellular tunnel edges via NMIIa driven actomyosin cable formation
- Caroline Stefani,
- David Gonzalez-Rodriguez,
- Yosuke Senju,
- Anne Doye,
- Nadia Efimova,
- Sébastien Janel,
- Justine Lipuma,
- Meng Chen Tsai,
- Daniel Hamaoui,
- Madhavi P. Maddugoda,
- Olivier Cochet-Escartin,
- Coline Prévost,
- Frank Lafont,
- Tatyana Svitkina,
- Pekka Lappalainen,
- Patricia Bassereau,
- Emmanuel Lemichez
Affiliations
- Caroline Stefani
- Department of microbial toxins in host-pathogen interactions, INSERM, U1065, Université de Nice-Sophia Antipolis, Centre Méditerranéen de Médecine Moléculaire (C3M)
- David Gonzalez-Rodriguez
- LCP-A2MC, Institut Jean Barriol, Université de Lorraine
- Yosuke Senju
- Program in Cell and Molecular Biology, Institute of Biotechnology, P.O. Box 56, University of Helsinki
- Anne Doye
- Department of microbial toxins in host-pathogen interactions, INSERM, U1065, Université de Nice-Sophia Antipolis, Centre Méditerranéen de Médecine Moléculaire (C3M)
- Nadia Efimova
- Department of Biology, University of Pennsylvania
- Sébastien Janel
- Multiscale Physics-Biology-Chemistry and Cancer, Cellular Microbiology and Physics of Infection Group, Center for Infection and Immunity of Lille, CNRS UMR8204, INSERM U1019, Institut Pasteur de Lille, Centre Hospitalier Régional de Lille, Université de Lille
- Justine Lipuma
- Department of microbial toxins in host-pathogen interactions, INSERM, U1065, Université de Nice-Sophia Antipolis, Centre Méditerranéen de Médecine Moléculaire (C3M)
- Meng Chen Tsai
- Department of microbial toxins in host-pathogen interactions, INSERM, U1065, Université de Nice-Sophia Antipolis, Centre Méditerranéen de Médecine Moléculaire (C3M)
- Daniel Hamaoui
- Department of microbial toxins in host-pathogen interactions, INSERM, U1065, Université de Nice-Sophia Antipolis, Centre Méditerranéen de Médecine Moléculaire (C3M)
- Madhavi P. Maddugoda
- Department of microbial toxins in host-pathogen interactions, INSERM, U1065, Université de Nice-Sophia Antipolis, Centre Méditerranéen de Médecine Moléculaire (C3M)
- Olivier Cochet-Escartin
- Multiscale Physics-Biology-Chemistry and Cancer, Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS, UMR168
- Coline Prévost
- Multiscale Physics-Biology-Chemistry and Cancer, Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS, UMR168
- Frank Lafont
- Multiscale Physics-Biology-Chemistry and Cancer, Cellular Microbiology and Physics of Infection Group, Center for Infection and Immunity of Lille, CNRS UMR8204, INSERM U1019, Institut Pasteur de Lille, Centre Hospitalier Régional de Lille, Université de Lille
- Tatyana Svitkina
- Department of Biology, University of Pennsylvania
- Pekka Lappalainen
- Program in Cell and Molecular Biology, Institute of Biotechnology, P.O. Box 56, University of Helsinki
- Patricia Bassereau
- Multiscale Physics-Biology-Chemistry and Cancer, Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS, UMR168
- Emmanuel Lemichez
- Department of microbial toxins in host-pathogen interactions, INSERM, U1065, Université de Nice-Sophia Antipolis, Centre Méditerranéen de Médecine Moléculaire (C3M)
- DOI
- https://doi.org/10.1038/ncomms15839
- Journal volume & issue
-
Vol. 8,
no. 1
pp. 1 – 14
Abstract
Holes in endothelial barriers, called transendothelial cell macroapertures (TEMs), are predicted to be limited by line tension of unknown origin. Here the authors identify an actomyosin cable encircling TEMs and establish a role for ezrin in stabilising F-actin bundles, allowing their crosslinking by non-muscle myosin IIa.
