Quantitative analysis of C. elegans transcripts by Nanopore direct-cDNA sequencing reveals terminal hairpins in non trans-spliced mRNAs

Florian Bernard; Delphine Dargère; Oded Rechavi; Denis Dupuy

doi:10.1038/s41467-023-36915-0

Nature Communications (Mar 2023)

Quantitative analysis of C. elegans transcripts by Nanopore direct-cDNA sequencing reveals terminal hairpins in non trans-spliced mRNAs

Florian Bernard,
Delphine Dargère,
Oded Rechavi,
Denis Dupuy

Affiliations

Florian Bernard: Université de Bordeaux, Inserm U1212, CNRS UMR5320, Institut Européen de Chimie et Biologie (IECB)
Delphine Dargère: Université de Bordeaux, Inserm U1212, CNRS UMR5320, Institut Européen de Chimie et Biologie (IECB)
Oded Rechavi: Department of Neurobiology, Wise Faculty of Life Sciences & Sagol School of Neuroscience, Tel Aviv University
Denis Dupuy: Université de Bordeaux, Inserm U1212, CNRS UMR5320, Institut Européen de Chimie et Biologie (IECB)

DOI: https://doi.org/10.1038/s41467-023-36915-0
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 11

Abstract

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C. elegans long read transcriptomic analysis provides evidence that non-trans-spliced mRNAs display a terminal a hairpin structure mimicking the Spliced Leader. This provides an explanation how the main maturation system might be bypassed.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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